Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using human pancreatic cancer cells. The ED 50 for this effect is 1-4 μg/mL.
Measured in a cell proliferation assay using EJG bovine adrenal-derived endothelial cells. The ED 50 for this effect is 1-4 µg/mL.Source
Mouse myeloma cell line, NS0-derived Ala20-Phe105
Accession #
N-terminal Sequence
AnalysisAla20
Predicted Molecular Mass
9.6 kDa
SDS-PAGE
9.6 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2464-EV/CF |
| 2464-EV |
Formulation Supplied as a 0.2 μm filtered solution in PBS. | Formulation Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: EG-VEGF/PK1
Endocrine gland-derived vascular endothelial cell growth factor (EG-VEGF; also called prokineticin-1) is a member of the prokineticin family of secreted proteins whose distinguishing structural motif involves 10 cysteines that form five intrachain disulfide bonds, creating a “colipase fold” (1, 2). The mouse EG‑VEGF precursor is 105 amino acids in length and contains a 19 aa signal sequence plus an 86 aa mature segment (3). There are no potential N‑linked glycosylation sites, and the secreted molecule is a monomer that is approximately 10 kDa in size. The mature region in mouse is 93% and 87% aa identical to the mature regions in rat and human, respectively. Mature mouse prokineticin-2 is 57% aa identical to mouse EG‑VEGF/prokineticin-1. Mouse EG‑VEGF is active on bovine endothelial cells (3). Although there is considerable aa identity between mouse and human EG-VEGF, the expression patterns are very much different. This is attributed to pronounced differences in the promoter region of the two genes. While human EG-VEGF is expressed by steroidogenic glands, mouse EG‑VEGF is expressed in hepatocytes and renal tubular epithelial cells. EG-VEGF binds to and activates two closely related G-protein coupled receptors, EG‑VEGF/PK1‑R1 and EG‑VEGF/PK2‑R2 (4, 5). Mouse EG‑VEGF stimulates proliferation and survival of liver sinusoidal endothelial cells, and, in mouse, EG-VEGF acts to induce monocyte migration and stimulate hematopoiesis (3, 6).
References:
LeCouter, J. et al. (2004) Ann. N.Y. Acad. Sci. 1014:50.
Li, M. et al. (2001) Mol. Pharmacol. 59:692.
LeCouter, J. et al. (2003) Endocrinology 144:2606.
Lin, D. et al. (2002) J. Biol. Chem. 277:19276.
Masuda, Y. et al. (2002) Biochem. Biophys. Res. Commun. 293:396.
LeCouter, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:16813.
Long Name:
Endocrine Gland-derived Vascular Endothelial Growth Factor
Entrez Gene IDs:
84432 (Human); 246691 (Mouse); 192205 (Rat)
Alternate Names:
EGVEGF; EG-VEGF; EGVEGFblack mamba toxin-related protein; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; PK1mambakine; PRK1prokineticin-1; PROK1; Prokineticin 1
