Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rmLayilin at 5 µg/mL (100 µL/well) can bind biotinylated hyaluronan with a linear range of 0.8-50 µg/mL.
Source
Mouse myeloma cell line, NS0-derived Lys18-Glu229 (Ala29-Gly36 del), with a C-terminal 10-His tag
Accession #
N-terminal Sequence
AnalysisLys18
Predicted Molecular Mass
24.8 kDa
SDS-PAGE
38 kDa, reducing conditions
2646-LA |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Layilin
Layilin (named after the L-A-Y-I-L-I amino acid motif in the transmembrane segment) is a member of the animal C-type lectin family (1). The mouse Layilin cDNA encodes a 402 amino acid (aa) type I transmembrane protein that, based on the orthologous molecule in hamster, contains a 19 aa signal sequence, a 211 aa extracellular domain (ECD), a 30 aa transmembrane segment, and a 141 aa cytoplasmic tail (2, 3). The ECD of mouse Layilin shares 85%, 70%, and 77% aa sequence identity with that of hamster, rat, and human Layilin, respectively. The ECD includes one carbohydrate recognition domain that contains a single potential Ca++-binding site (3). The intracellular region contains three types of LH (Layilin Homology) repeats: three 16 - 18 aa LH1 repeats, three 5 aa LH2 repeats, and one 4 aa LH3 repeat (3). Adjacent LH2-LH3 tandem arrays provide docking sites for the actin cytoskeleton adaptor proteins talin, merlin, and radixin (3, 4, 5). Layilin interacts with the ubiquitous extracellular matrix component hyaluronan but not with heparin, chondroitin sulfate, or N-acetyl glucosamine (6). Layilin is widely expressed and localizes to ruffling edges in spreading and migrating cells (3, 5). It does not localize to focal contacts and does not interact with the focal contact proteins alpha ‑actinin, vinculin, tensin, paxillin, tubulin, or Integrin beta 1 (3).
References:
McGreal, E.P. et al. (2004) Mol. Immunol. 41:1109.
Genbank Accession # XP_146887.
Borowsky, M.L. and R.O. Hynes (1998) J. Cell Biol. 143:429.
Rees, D.J.G. et al. (1990) Nature 347:685.
Bono, P. et al. (2005) Exp. Cell Res. 308:177.
Bono, P. et al. (2001) Mol. Biol. Cell 12:891.
Entrez Gene IDs:
143903 (Human); 244864 (Mouse)
Alternate Names:
FLJ30977; FLJ31092; Layilin; LAYN; LYAN
