Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IFN-gamma secretion by NK‑92 human natural killer lymphoma cells. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495. The ED 50 for this effect is 0.6-3 μg/mL.
Source
Mouse myeloma cell line, NS0-derived
Human B7-H6
(Met1 - Ser262)
Accession # NP_001189368IEGRMD Human IgG1
(Pro100 - Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisAsp25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
53.3 kDa (monomer)
SDS-PAGE
75-85 kDa, reducing conditions
7144-B7 |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Data Images
Detection of NKp30 with RecombinantHuman B7-H6 Fc Chimera Protein by FlowCytometry.
| HEK293 human embryonic kidney cell line transfected withhuman NKp30 and eGFP was stained with (A) Mouse Anti-human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A) alone, or (B) Recombinant Human B7-H6 Fc chimeraprotein (Catalog # 7144-B7) followed by Mouse Anti-human IgG Fc APC-conjugated Monoclonal Antibody. |
Background: B7-H6
B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins (1, 2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53% - 56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7).
References:
Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
Li, Y. et al. (2011) J. Exp. Med. 208:703.
Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. 108:6223.
Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
Byrd, A. et al. (2007) PLoS ONE 2:e1339.
Long Name:
B7 Homolog 6
Entrez Gene IDs:
374383 (Human); 102121659 (Cynomolgus Monkey)
Alternate Names:
B7 homolog 6; B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1
