Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human His6-alpha -Synuclein is ideal for use as a control substrate for in vitro Ubiquitin conjugation using select Ubiquitin E3 ligases such as CHIP/Stub1. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-alpha -Synuclein concentration of 0.5-2.5 μM.
Source
E. coli-derived Contains a C-terminal 6-His tag
Accession #
Predicted Molecular Mass
15 kDa
SP-480 |
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Formulation Supplied as a solution in HEPES and NaCl. | ||
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: alpha-Synuclein
alpha -Synuclein is member of a family of small soluble proteins that include also beta -, and gamma -Synuclein. It is predominantly expressed in neurons of the central nervous system in the presynaptic region of nerve terminals, where cycles between free partially unfolded and helical membrane-bound forms. alpha -Synuclein can self-aggregate in vivo and in vitro, forming various oligomeric species and fibrillar and amorphous aggregates. The fibrils and amyloidal forms of alpha -Synuclein are major components of Lewy bodies and Lewy neurites and have been linked to the pathogenesis of Parkinson’s disease, Parkinson’s disease dementia, and dementia with Lewy bodies. alpha -Synuclein aggregates can be also found associated with amyloid plaques in Alzheirmer’s disease.
References:
Breydo L, Wu J.W., Uversky V.N. (2012) Biochim Biophys Acta. 1822: 261
Chen R.H., et al. (2013) J Biol Chem. 288: 7438
Li X., et al. (2008) Acta Biochim Biophys Sin (Shanghai) 40: 406
Surguchov A. (2008) Int Rev Cell Mol Biol 270: 225
Xia Q., et al. (2008) Front Biosci. 13: 3850
Entrez Gene IDs:
6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)
Alternate Names:
alpha-Synuclein; Lewy body) 4; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; non-A4 component of amyloid; PARK1; PARK4; PD1; SNCA; synuclein, alpha (non A4 component of amyloid precursor); Synuclein-alpha
