•Highdynamicrange(signalupto60millioncounts/s)
测试实例展示Alba-STEDforFLIM/FFS
Confocal(left)vs.pSTED(right)imagesoftheactinlabeledwiththeSiRdyeinfixedgliacells,acquiredbyFastFLIM.
Confocal(left)vs.pSTED(right)imagesoftheactinlabeledwiththeSiRdyeinfixedgliacells,acquiredbyFastFLIM.
Confocalimagesof60nmfluorescencebeads(left);pSTEDimages(middle);sharpeningthepSTEDimageusingabinaryfilterbasedonthephasorplots(right).
DuallabelscanbeseparatedusingpSTEDandFastFLIM.Atto647NandAtto655wereusedaslabels;theybothareexcitedbythe640nmlaser.Thetwodyesarefirstseparatedusingthephasorplots,andthenassignedwithtwodifferentfalsecolors(Atto647N-yellow,Atto655-purple)toproducetheprocessedandmergedpSTEDimageofthetwolabels.
参考论文：
Monomericcohesinstaterevealedbylive-cellsingle-moleculespectroscopy.
Liu,W.,Biton,E.,Pathania,A.,Matityahu,A.,Irudayaraj,J.,Onn,I.
EMBORep.2019Dec29:e48211.doi:10.15252/embr.201948211.[Epubaheadofprint]
Photon-separationtoenhancethespatialresolutionofpulsedSTEDmicroscopy.
Tortarolo,G.,Sun,Y.,Teng,K.W.,Ishitsuka,Y.,Lanzanó,L.,Selvin,P.R.,Barbieri,B.,Diaspro,A.,Vicidomini,G.
Nanoscale.2019Jan9.doi:10.1039/c8nr07485b.[Epubaheadofprint]
AstraightforwardSTED-backgroundcorrectedfittingmodelforunbiasedSTED-FCSanalyses.
Wang,R.,Brustlein,S.,Mailfert,S.,Fabre,R.,Fallet,M.,Sivankutty,S.,Rigneault,H.,Marguet,D.
Methods.2018May1;140-141:212-222.doi:10.1016/j.ymeth.2018.02.010.Epub2018Feb14.
ANovelPulsedSTEDMicroscopyMethodUsingFastFLIMandthePhasorPlots
Sun,Y.,TortaroloG.,Teng,K.-W.,Ishitsuka,Y.,Coskun,U.C.,Liao,S.-C.,Diaspro,A.,Vicidomini,G.,Selvin,P.R.,Barbieri,B.
Proc.SPIE10069,MultiphotonMicroscopyintheBiomedicalSciencesXVII,100691C(February21,2017)
