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• Brand

• BD Lyoplate™

• Regulatory Status

• RUO

Regulatory Status Legend

• RUO

• For Research Use Only. Not for use in diagnostic or therapeutic procedures.

• RUO (GMP)

• For Research Use Only. Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

• GPR

• General Purpose Reagent

• IVD

• For In Vitro Diagnostic Use.

• ASR

• Analyte Specific Reagent. Analytical and performance characteristics are not established.

Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.

Description

The BD Lyoplate™ Mouse Cell Surface Marker Screening Panel contains 176 purified monoclonal antibodies specific for different cell surface markers. The panel also contains rat, mouse and hamster immunoglobulin (Ig) isotype controls for assessing nonspecific background staining. The panel can be used for screening cell lines, primary cells or tissues and is compatible with flow cytometry and bioimaging technology platforms. The panel contains three 96-well plates with antibody-containing wells having 2.75 µg of primary antibody each (enough for five tests at 0.5 µg of antibody/test). Biotinylated secondary anti-Ig antibodies and AlexaFluor® 647-conjugated Streptavidin as the tertiary reagent are also included in the panel. This product is compatible with cells that express fluorescent reporter genes, such as green fluorescent protein (GFP). It can be used with additional antibodies that recognize cell surface and intracellular molecules. Positive hits from screens can be followed up with subsequent confirmation experiments using either purified or fluorescent antibodies offered by BD Biosciences. To access this content, you can search for either the antibody clone name and/or the name of the specificity on the BD Biosciences website: http://www.bdbiosciences.com.

Component 51-9007606AK - Mouse Cell Surface Marker Panel - Part A

· Mouse Cell Surface Marker Lyoplate Plate 1 (1 each)

· Mouse Cell Surface Marker Lyoplate Plate 2 (1 each)

· Mouse Cell Surface Marker Lyoplate Plate 3 (1 each)

Storage: Store unopened plates at room temperature (18-25°C).

         Antibodies were lyophilized in an aqueous buffered solution containing BSA and ≤ 0.09% sodium azide.

Component 51-9007606BK - Mouse Cell Surface Marker Screening Panel - Part B

· Biotin Goat Anti-Rat Ig (1.0 ml)

· Biotin Goat Anti-Mouse Ig (0.25 ml)

· Biotin Goat Anti-Armenian Hamster Ig (0.25 ml)

· Biotin Goat Anti-Syrian Hamster Ig (0.1 ml)

· Alexa Fluor® 647 Streptavidin (0.2 ml)

Storage: Store the biotinylated secondary antibodies and the fluorescent streptavidin protected from light at 4°C.

The biotinylated secondary antibodies are provided in an aqueous buffered solution containing ≤ 0.09% sodium azide.

Note that antibodies present in this panel may not recognize all isoforms of each cell surface marker. In addition, antibody clones can behave differently on cell types depending on the availability of epitopes present, ie, certain epitopes can be occluded by post-translational modifications. Results you obtain in this screen may only be relevant to the antibody clones tested. Moreover, since all the antibodies are provided at the same fixed amounts, they may or may not be at their optimal concentrations. Therefore, it is important to verify positive screening hits with either purified or fluorescent antibodies that are used at optimal concentrations (determined by titration) for result confirmation.

Product Notices

1. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).

2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

4. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.

5. Accutase is a registered trademark of Innovative Cell Technologies, Inc.

6. Alexa Fluor® is a registered trademark of Life Technologies Corporation.