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• Brand

• BD Lyoplate™

• Regulatory Status

• RUO

Regulatory Status Legend

• RUO

• For Research Use Only. Not for use in diagnostic or therapeutic procedures.

• RUO (GMP)

• For Research Use Only. Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

• GPR

• General Purpose Reagent

• IVD

• For In Vitro Diagnostic Use.

• ASR

• Analyte Specific Reagent. Analytical and performance characteristics are not established.

Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.

Description

The BD Lyoplate™ Human Cell Surface Marker Screening Panel contains 242 purified monoclonal antibodies to cell surface markers. The panel also contains both mouse and rat isotype controls for assessing isotype-specific background. The panel can be used for screening cell lines, primary cells or tissue, and is compatible with flow cytometry and bioimaging technology platforms. The panel contains three (3) 96 well plates, each well containing 2.75 µg of antibody, enough for five tests (0.5 µg/test) along with AlexaFluor® 647 conjugated goat anti-mouse Ig and goat anti-rat Ig secondary antibodies. This product is compatible with cells expressing fluorescent reporter genes, such as green fluorescent protein (GFP) and can be used with additional antibodies that recognize cell surface and intracellular molecules. Positive hits from screens can be followed-up with either purified or flurochrome-conjugated antibodies offered by BD Biosciences. To access this content, you can search either the name of the clone and/or the name of the specificity on our website: http://www.bdbiosciences.com

Component 51-9006585AK - Human Cell Surface Marker Panel - Part A

        ·        Human Cell Surface Marker Lyoplate Plate 1 (1 each)

        ·        Human Cell Surface Marker Lyoplate Plate 2 (1 each)

        ·        Human Cell Surface Marker Lyoplate Plate 3 (1 each)

        ·        Store unopened plates at room temperature (18-25°C).

        ·        Antibodies are lyophilized in an aqueous buffered solution containing BSA and ≤ 0.09% sodium azide.

Component 51-9006588BK - Human Cell Surface Marker Screening Panel - Part B

        ·        AlexaFluor® 647 Goat Anti-Mouse Ig (1.8 ml)

        ·        AlexaFluor® 647 Goat Anti-Rat Ig (0.6 ml)

        ·        Store the secondary antibodies at 4°C.

        ·        The secondary antibodies are provided in an aqueous buffered solution containing ≤ 0.09% sodium azide.

It is important to note the antibodies present in this panel may not recognize all isoforms of each cell surface marker. In addition, antibody clones can behave differently on cell types depending on the availability of epitopes present, i.e., certain epitopes can be occluded by post-translational modifications. Results you obtain in this screen may only be relevant to the antibody clones tested.

Product Notices

1. Alexa Fluor® is a registered trademark of Life Technologies Corporation.

2. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.

3. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).

4. This product may be covered by US Patent No. 5,543,320.

5. US Patent No. 5,994,515, University of Pennsylvania.

6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.