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• Brand

• BD Pharmingen™

• Regulatory Status

• RUO

Regulatory Status Legend

• RUO

• For Research Use Only. Not for use in diagnostic or therapeutic procedures.

• RUO (GMP)

• For Research Use Only. Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

• GPR

• General Purpose Reagent

• IVD

• For In Vitro Diagnostic Use.

• ASR

• Analyte Specific Reagent. Analytical and performance characteristics are not established.

Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.

Description

Protective immunity depends on coordinated responses between regulatory and conventional T cells and other leucocyte subsets. High resolution flow cytometric analyses of signaling responses made by leucocyte subsets, defined by their coexpressed levels of surface differentiation antigens and specific transcription factors, provides deep insights into the cellular and molecular mechanisms that underlie immune regulation in healthy and diseased states. Historically, disparate buffer compatibility characteristics have created challenges for the simultaneous analysis of these coexpressed biomarkers. The BD Pharmingen™ Transcription Factor Phospho (TFP) Buffer Set is designed to enable robust and simultaneous flow cytometric analysis of various cell surface antigens, transcription factors, and phosphorylated signal proteins in cells derived from both human and mouse tissues. This includes fresh, fixed, and frozen human peripheral blood mononuclear cells.

The BD Pharmingen™ TFP Buffer Set has been successfully applied to the multiparameter flow cytometric analysis of phosphorylated Stat signaling responses to cytokines made by human and mouse T regulatory cell and conventional T cell subsets, and other leucocyte types defined by certain cell surface markers, transcription factors (FoxP3 and T-bet), and physical characteristics. While not all antibody compatibilities are known, cell signaling responses to different stimuli should also be amenable to analysis using this buffer set and antibodies specific for other cell surface markers, transcription factors, and types of downstream phosphorylated signaling proteins. This buffer system is backwards compatible with many BD reagents, including antibodies conjugated with new fluorescent dyes (eg, BD Horizon™ BV421, BV510, PE-CF594, V450, and V500).

4X TFP Fix/Perm Buffer - Contains 15.6% formaldehyde, 5.5% methanol

Hazard statements

Combustible liquid. Harmful if swallowed or in contact with skin. Toxic if inhaled.

Causes skin irritation. Causes serious eye damage. May cause an allergic skin reaction.

Suspected of causing genetic defects. May cause cancer. Route of exposure: Inhalative.

May cause damage to the central nervous system. Route of exposure: Oral. May cause respiratory irritation.

Precautionary statements

Wear protective gloves / eye protection. Wear protective clothing. Do not breathe mist/vapours/spray.

IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell

Perm Buffer III - Contains 88% methanol

Hazard statements

Highly flammable liquid and vapor. Toxic if swallowed, in contact with skin or if inhaled.

Causes damage to the central nervous system. Route of exposure: Oral.

Precautionary statements

Keep away from heat/sparks/open flames/hot surfaces. - No smoking.

Wear protective clothing / eye protection. Wear protective gloves. Do not breathe mist/vapours/spray.

IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.

Product Notices

1. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.

2. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.

3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

5. Cy is a trademark of GE Healthcare.