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| Description | |
|---|---|
| Overview | Dimeric, native MMP-9 from stimulated human neutrophils. Enzyme should be activated just prior to use. |
| M.W. 222,000 (non-reducing SDS-PAGE). Note: 1 mU = 1 milliunit. | |
| Catalogue Number | 444232 |
| Brand Family | Calbiochem® |
| Synonyms | Matrix Metalloproteinase 9, Gelatinase B, 92 kDa type IV Collagenase |
| References | |
|---|---|
| References | Olson, M.W. et al. 2000. J. Biol. Chem. 275, 2661. Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529. |
| Product Information | |
|---|---|
| Unit of Definition | One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0. |
| EC number | 3.4.24.35 |
| Form | Liquid |
| Formulation | In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ® 35 Detergent, 0.05% NaN₃, pH 7.0. |
| Applications |
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| Biological Information | |
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| Purity | ≥90% by SDS-PAGE |
| Source | Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. |
| Specific Activity | ≥1000 mU/mg protein |
| Physicochemical Information | |
|---|---|
| Contaminants | No other MMP activity detectable |
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| Safety Information according to GHS |
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| Product Usage Statements |
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| Storage and Shipping Information | |
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| Ship Code | Dry Ice Only |
| Toxicity | Standard Handling |
| Storage | ≤ -70°C |
| Avoid freeze/thaw | Avoid freeze/thaw |
| Do not freeze | Ok to freeze |
| Special Instructions | Following initial thaw, aliquot and freeze (-70°C). |
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