详细说明
| Replacement Information |
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重要规格表
| Detection Methods |
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| Colorimetric |
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| Description | |
|---|---|
| Overview | A sensitive spectrophotometric assay (340 nm) for the measurement of glutathione peroxidase (GPx) activity in plasma, erythrocyte lysates, tissue homogenates, and cell lysates. Assay range: 50-344 nmol/min/ml. Contains sufficient reagents for 96 tests. |
| Catalogue Number | 353919 |
| Brand Family | Calbiochem® |
| Materials Required but Not Delivered | • A plate reader with a 340 nm filter • Adjustable pipettors and a repeat pipettor • A source of pure water. Glass distilled water or HPLC-grade water is acceptable • PBS • Homogenization buffer (cell and tissue preparation) |
| References | |
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| References | Ursini, F., et al. 1985. Biochim. Biophys. Acta 839, 62. Forstrom, J.W., et al. 1978. Biochem. 17, 2639. Paglia, D.E., et al. 1967. J. Lab. Clin. Med. 70, 158. |
| Product Information | |
|---|---|
| Detection method | Colorimetric |
| Form | 96 Tests |
| Format | 96-well plate |
| Kit contains | Assay Buffer, Sample Buffer, Glutathione Peroxidase (Control), Co-Substrate Mixture, Cumene Hydroperoxide, 96 Well Plate, Plate Cover, and a user protocol. |
| Positive control | Bovine erythrocyte glutathione peroxidase |
| Applications |
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| Biological Information | |
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| Assay range | 50-344 nmol/min/ml |
| Assay time | 2 h |
| Sample Type | Cells, tissues, plasma |
| Physicochemical Information |
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| Dimensions |
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| Materials Information |
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| Toxicological Information |
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| Safety Information according to GHS |
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| Safety Information |
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| Product Usage Statements |
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| Storage and Shipping Information | |
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| Ship Code | Blue Ice Only |
| Toxicity | Standard Handling |
| Storage | -20°C |
| Storage Conditions | Upon arrival store the entire contents of the kit at -20°C. |
| Do not freeze | Ok to freeze |
| Packaging Information |
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| Transport Information |
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| Supplemental Information | |
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| Kit contains | Assay Buffer, Sample Buffer, Glutathione Peroxidase (Control), Co-Substrate Mixture, Cumene Hydroperoxide, 96 Well Plate, Plate Cover, and a user protocol. |
| Specifications |
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