详细说明
点击查看资料
中文名:KM培养基基盐/Kao & Michayluk培养基基盐(不含维生素)
英文名:Kao & Michayluk Base Salts
CAS:N/A
级别:N/A
分子量:N/A
分子式:N/A
纯度:N/A
储存条件:2-8℃
产品简介
产品性状:白色至淡黄色粉末
工作浓度:水溶解,3622.59mg/L
运输条件:常温运输
储存条件:2-8℃,密封储存
特别说明:该产品不适宜制备高浓度母液,会有沉淀产生。
产品组成:(mg/L)
产品应用:根据需要额外添加碳源(蔗糖等)、凝胶(琼脂、植物凝胶等)、植物激素等,用于植物组织培养实验。
特别推荐:Coolaber公司可根据需求定制植物培养基,起订量仅1kg,详情请咨询销售人员,咨询电话400-878-6800。
The medium defined by Kao and Michayluk was designed to grow cells and protoplasts of Vicia hajastana at a very low population -density in liquid media. The inability of the plant cells to grow at a very low population density may be caused by excessive diffusion of metabolic intermediates into the medium, resulting in their dilution in the cell to a level below that required for survival. Vicia cells were able to grow at an initial population density of 1-2 cells/ml when the mineral salt medium was enriched with organic acids, sugars, sugar alcohols, growth regulators, amino acids and other organic compounds. The percentage of cell division could be increased by raising the -concentration of CaCl2 from 1 mM, as in Gamborg B5, to 5 mM. Calcium may play an important role in the proces of cell division because of its ability to preserve the structural and functional integrity of plant cell membranes. The medium defined by Kao and Michayluk was designed to grow cells and protoplasts of Vicia hajastana at a very low population -density in liquid media. The inability of the plant cells to grow at a very low population density may be caused by excessive diffusion of metabolic intermediates into the medium, resulting in their dilution in the cell to a level below that required for survival. Vicia cells were able to grow at an initial population density of 1-2 cells/ml when the mineral salt medium was enriched with organic acids, sugars, sugar alcohols, growth regulators, amino acids and other organic compounds. The percentage of cell division could be increased by raising the -concentration of CaCl2 from 1 mM, as in Gamborg B5, to 5 mM. Calcium may play an important role in the proces of cell division because of its ability to preserve the structural and functional integrity of plant cell membranes.