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详细说明
概述
产品名称Anti-2 Hydroxy phytanoyl CoA lyase抗体[EPR16812]
描述
兔单克隆抗体[EPR16812] to 2 Hydroxy phytanoyl CoA lyase
经测试应用WB,IP,Flow Cyt
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Human 2 Hydroxy phytanoyl CoA lyase aa 200-400. The exact sequence is proprietary.
Database link: Q9UJ83Run BLAST with
Run BLAST with 
阳性对照
HeLa, 293T, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, Mouse heart, Rat brain and Rat heart lysates; 293T cells; HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR16812
同种型IgG
研究领域
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Lipid metabolism
Cancer
Cancer Metabolism
Metabolic signaling pathway
Metabolism of lipids and lipoproteins
Tags & Cell Markers
Subcellular Markers
Organelles
Peroxisome
Anti-2 Hydroxy phytanoyl CoA lyase antibody [EPR16812] 图像
![Western blot - Anti-2 Hydroxy phytanoyl CoA lyase antibody [EPR16812] (ab197025)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab197025025.jpg)
Western blot - Anti-2 Hydroxy phytanoyl CoA lyase antibody [EPR16812] (ab197025)
All lanes : Anti-2 Hydroxy phytanoyl CoA lyase antibody [EPR16812] (ab197025) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 64 kDa
Observed band size : 64 kDa
Exposure time : 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-2 Hydroxy phytanoyl CoA lyase antibody [EPR16812] (ab197025)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab197025252.jpg)
Western blot - Anti-2 Hydroxy phytanoyl CoA lyase antibody [EPR16812] (ab197025)
All lanes : Anti-2 Hydroxy phytanoyl CoA lyase antibody [EPR16812] (ab197025) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat heart lysate
Lane 5 : C6 (Rat glial tumor cells) whole cell lysates
Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 8 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 64 kDa
Observed band size : 64 kDa
Exposure time : 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
![Immunoprecipitation - Anti-2 Hydroxy phytanoyl CoA lyase antibody [EPR16812] (ab197025)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1970255IP.jpg)
Immunoprecipitation - Anti-2 Hydroxy phytanoyl CoA lyase antibody [EPR16812] (ab197025)
2 Hydroxy phytanoyl CoA lyase was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab197025 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab197025 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract (Input) 10 µg. Lane 2: ab197025 IP in HeLa whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab197025 in HeLa whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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