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详细说明
概述
产品名称Anti-ApoER2抗体[EPR3326]
描述
兔单克隆抗体[EPR3326] to ApoER2
经测试应用WB,IP
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ApoER2 aa 950 to the C-terminus (C terminal).
Database link: Q14114
(Peptide available as ab193569)阳性对照
SH-SY5Y, C6, Neuro-2a, Human fetal brain, Mouse brain, and Rat brain lysates,
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS纯度Protein A purified
克隆单克隆
克隆编号EPR3326
同种型IgG
研究领域
Metabolism
Types of disease
Cancer
Metabolism
Types of disease
Obesity
Cardiovascular
Lipids / Lipoproteins
Lipoproteins/Apolipoproteins
Apolipoproteins
Signal Transduction
Metabolism
Lipid metabolism
Neuroscience
Neurology process
Neurogenesis
Neuroscience
Neurology process
Neurodegenerative disease
Other
Anti-ApoER2 antibody [EPR3326] 图像
![Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab10/ab108208WB1.jpg)
Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/10000 dilution (purified)
Lane 1 : SH-SY5Y (human neuroblastoma) whole cell lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : C6 (rat glioma) whole cell lysate
Lane 5 : Neuro-2a (mouse neuroblastoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size : 106 kDa
Observed band size : 130-170 kDa (why is the actual band size different from the predicted?)
Blocking and diluting buffer 5% NFDM/TBST.
130kDa: Immature form;
170kDa: Glycosylated mature form. (PMID:24344333, 24532792, 24705369 and 25233900)
![Western blot - ApoER2 antibody [EPR3326] (ab108208)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab10/ab1082088-2.JPG)
Western blot - ApoER2 antibody [EPR3326] (ab108208)
All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/1000 dilution (unpurified)
Lane 1 : SH-SY5Y cell lysate
Lane 2 : C6 cell lysate
Lane 3 : Neuro-2a cell lysate
Lane 4 : Human Fetal Brain cell lysate
Lane 5 : Mouse Fetal Brain cell lysate
Lane 6 : Rat Fetal Brain cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 106 kDa
![Immunoprecipitation - Anti-ApoER2 antibody [EPR3326] (ab108208)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab10/ab108208IP1.jpg)
Immunoprecipitation - Anti-ApoER2 antibody [EPR3326] (ab108208)
ab108208 (purified) at 1/40 immunoprecipitating ApoER2 in SH-SY5Y (human neuroblastoma) whole cell lysate.
Lane 1 (input): SH-SY5Y whole cell lysate 10ug
Lane 2 (+): ab108208 + SH-SY5Y whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108208 in SH-SY5Y whole cell lysate
For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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