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详细说明
概述
产品名称Anti-ATP5G1/G2/G3抗体[EPR13908]
描述
兔单克隆抗体[EPR13908] to ATP5G1/G2/G3
经测试应用WB,IHC-P,IP
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATP5G1/G2/G3 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: P05496阳性对照
HeLa and Human fetal heart lysates; Human liver tissue.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR13908
同种型IgG
研究领域
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Oxidative phosphorylation
Complex V
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Mitochondrial markers
Signal Transduction
Metabolism
Mitochondrial
Anti-ATP5G1/G2/G3 antibody [EPR13908] 图像
![Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180149WB2.jpg)
Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
All lanes : Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution (purified)
Lane 1 : mouse heart lysate
Lane 2 : rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size : 14 kDa
Observed band size : 8 kDa (why is the actual band size different from the predicted?)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST![Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180149WB1.jpg)
Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution (purified) + human fetal heart lysate at 10 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size : 14 kDa
Observed band size : 8 kDa (why is the actual band size different from the predicted?)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180149HC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
Immunohistochemical staining of paraffin embedded human hepatocellular carcinoma with purified ab180149 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1801499WB.jpg)
Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
All lanes : Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size : 14 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180149IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
Immuohistochemical analysis of Paraffin-embedded Human liver tissue labeling ATP5G1 with unpurified ab180149 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.
![Immunoprecipitation - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1801499IP.jpg)
Immunoprecipitation - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)
Western blot analysis of fetal heart lysate immunoprecipitated with unpurified ab180149 at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
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