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详细说明
概述
产品名称Anti-DUSP16抗体[EPR13583]
描述
兔单克隆抗体[EPR13583] to DUSP16
经测试应用WB,IHC-P,Flow Cyt
种属反应性
与反应: Mouse, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DUSP16 aa 600 to the C-terminus. The exact sequence is proprietary.
Database link: Q9BY84阳性对照
293 and HepG2 cell lysates; Human breast carcinoma and kidney tissues; HepG2 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR13583
同种型IgG
研究领域
Signal Transduction
Protein Phosphorylation
Ser / Thr Kinases
MAPK Pathway
Anti-DUSP16 antibody [EPR13583] 图像
![Flow Cytometry - Anti-DUSP16 [EPR13583] antibody (ab181088)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18108888d.jpg)
Flow Cytometry - Anti-DUSP16 [EPR13583] antibody (ab181088)
Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling DUSP16 with ab181088 at 1/270 dilution (red) compared to a rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP16 [EPR13583] antibody (ab181088)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181088HCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP16 [EPR13583] antibody (ab181088)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DUSP16 with ab181088 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP16 [EPR13583] antibody (ab181088)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181088IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP16 [EPR13583] antibody (ab181088)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling DUSP16 with ab181088 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
![Western blot - Anti-DUSP16 [EPR13583] antibody (ab181088)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1810888WB.jpg)
Western blot - Anti-DUSP16 [EPR13583] antibody (ab181088)
All lanes : Anti-DUSP16 antibody [EPR13583] (ab181088) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 73 kDa
Observed band size : 79 kDa (why is the actual band size different from the predicted?)
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