概述

• 产品名称Anti-FNTB抗体[EPR4706]
     

• 描述

  兔单克隆抗体[EPR4706] to FNTB

• 经测试应用ICC/IF,WB

• 种属反应性

  与反应: Rat, Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human FNTB aa 1-100 (N terminal).

• 阳性对照    

• WB: JAR, HepG2, K562, SH-SY5Y and C6 cell lysates. ICC/IF: K562 cells.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR4706

• 同种型IgG

• 研究领域

• Metabolism

• Types of disease

• Cancer

• Metabolism

• Pathways and Processes

• Metabolic signaling pathways

• Amino acid metabolism

• <a javascript:void(0)>Signal Transduction

• Metabolism

• Amino Acids

Anti-FNTB antibody [EPR4706] 图像

• 

  Western blot - Anti-FNTB antibody [EPR4706] (ab109748)          

     Anti-FNTB antibody [EPR4706] (ab109748) at 1/5000 dilution (purified) + C6 whole cell lysate at 10 µg
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
  
  Predicted band size : 49 kDa
  Observed band size : 46 kDa (why is the actual band size different from the predicted?)
     

  Blocking and dilution buffer: 5% NFDM/TBST.

   

• 

  Western blot - Anti-FNTB antibody [EPR4706] (ab109748)          

     All lanes : Anti-FNTB antibody [EPR4706] (ab109748) at 1/5000 dilution (purified)
  
  Lane 1 : JAR whole cell lysate
  Lane 2 : HepG2 whole cell lysate
  Lane 3 : K562 whole cell lysate
  Lane 4 : SH-SY5Y whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
  
  Predicted band size : 49 kDa
  Observed band size : 46 kDa (why is the actual band size different from the predicted?)
     

  Blocking and dilution buffer: 5% NFDM/TBST.

   

• 

  Immunocytochemistry/ Immunofluorescence - Anti-FNTB antibody [EPR4706] (ab109748)          

     

  Immunocytochemistry/Immunofluorescence analysis of K562 cells labelling FNTB with purified ab109748 at 1/500. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

  Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).

  Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).

   

• 

  Western blot - FNTB antibody [EPR4706] (ab109748)          

     All lanes : Anti-FNTB antibody [EPR4706] (ab109748) at 1/1000 dilution (unpurified)
  
  Lane 1 : JAR cell lysate
  Lane 2 : HepG2 cell lysate
  Lane 3 : K562 cell lysate
  Lane 4 : SH-SY5Y cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 49 kDa