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详细说明
概述
产品名称Anti-Caveolin-2抗体[EPR5471]
描述
兔单克隆抗体[EPR5471] to Caveolin-2
经测试应用WB,ICC/IF
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caveolin-2 (C terminal).
Database link: P51636阳性对照
HUVEC, HeLa, 3T3-L1, and U87-MG cell lysates.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A trial size is available to purchase for this antibody.
性能
形式Liquid
存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR5471
同种型IgG
研究领域
Cardiovascular
Atherosclerosis
Apoptosis
Cardiovascular
Atherosclerosis
Lipid transport
Tags & Cell Markers
Subcellular Markers
Organelles
Caveolae and Clathrin
Anti-Caveolin-2 antibody [EPR5471] 图像
![Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (ab133484)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab13/ab133484100.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (ab133484)
ab133484 stained MCF cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133484 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
![Western blot - Anti-Caveolin-2 [EPR5471] antibody (ab133484)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab13/ab1334844-1.jpg)
Western blot - Anti-Caveolin-2 [EPR5471] antibody (ab133484)
All lanes : Anti-Caveolin-2 antibody [EPR5471] (ab133484) at 1/1000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 3T3-L1 cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 18 kDa
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