概述

• 产品名称Anti-C2抗体[EPR17979]
     

• 描述

  兔单克隆抗体[EPR17979] to C2

• 经测试应用WB

• 种属反应性

  与反应: Mouse, Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human C2 aa 700 to the C-terminus. The exact sequence is proprietary.
  Database link: P06681

• 阳性对照    

• WB: Human serum, plasma and fetal kidney lysate; Mouse serum.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam's RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR17979

• 同种型IgG

• 研究领域

• Immunology

• Innate Immunity

• Complement

• Classical Pathway

Anti-C2 antibody [EPR17979] 图像

• 

  Western blot - Anti-C2 antibody [EPR17979] (ab209900)          

     All lanes : Anti-C2 antibody [EPR17979] (ab209900) at 1/1000 dilution
  
  Lane 1 : Human serum
  Lane 2 : Human plasma
  Lane 3 : Human fetal kidney lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Lanes 1 - 2 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
  Lane 3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution
  
  
  Predicted band size : 83 kDa
  Observed band size : 100 kDa (why is the actual band size different from the predicted?)
     

  Blocking/Dilution buffer: 5% NFDM/TBST.

  Exposure time: Lane 1/3: 3 minutes; Lane 2: 30 seconds.

   

• 

  Western blot - Anti-C2 antibody [EPR17979] (ab209900)          

     Anti-C2 antibody [EPR17979] (ab209900) at 1/1000 dilution + Mouse serum at 10 µg
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Predicted band size : 83 kDa
  Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 1 minute    

  Blocking/Dilution buffer: 5% NFDM/TBST.