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详细说明
概述
产品名称Anti-Germinal Center Kinase抗体[EPR14304]
描述
兔单克隆抗体[EPR14304] to Germinal Center Kinase
经测试应用Flow Cyt,IHC-P,WB
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Germinal Center Kinase aa 750 to the C-terminus. The exact sequence is proprietary.
Database link: Q12851阳性对照
Namalwa, Ramos and Human fetal brain lysates; Human hepatocellular carcinoma tissue; Ramos cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR14304
同种型IgG
研究领域
Signal Transduction
Protein Phosphorylation
Ser / Thr Kinases
Other Kinases
Signal Transduction
Protein Phosphorylation
Ser / Thr Kinases
MAPK Pathway
Anti-Germinal Center Kinase antibody [EPR14304] 图像
![Western blot - Anti-Germinal Center Kinase [EPR14304] antibody (ab184169)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184169-wb.jpg)
Western blot - Anti-Germinal Center Kinase [EPR14304] antibody (ab184169)
All lanes : Anti-Germinal Center Kinase antibody [EPR14304] (ab184169) at 1/10000 dilution
Lane 1 : Namalwa cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 92 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Germinal Center Kinase [EPR14304] antibody (ab184169)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184169ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Germinal Center Kinase [EPR14304] antibody (ab184169)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma labeling Germinal Center Kinase with ab184169 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
![Flow Cytometry - Anti-Germinal Center Kinase [EPR14304] antibody (ab184169)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184169-FC.jpg)
Flow Cytometry - Anti-Germinal Center Kinase [EPR14304] antibody (ab184169)
Flow cytometric analysis of Ramos cells (2% paraformaldehyde-fixed) labeling Germinal Center Kinase with ab184169 at 1/170 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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