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详细说明
概述
产品名称Anti-FXYD3抗体[EPR17160]
描述
兔单克隆抗体[EPR17160] to FXYD3
经测试应用WB,IHC-P,ICC/IF,Flow Cyt
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human FXYD3 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q14802阳性对照
WB: Human stomach and colon cancer lysates; MCF7 whole cell lysate. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa and HT-29 cells. Flow Cyt: MCF7 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR17160
同种型IgG
研究领域
Cancer
Tumor biomarkers
Other
Signal Transduction
Metabolism
Plasma Membrane
Channels
Signal Transduction
Metabolism
Plasma Membrane
ATPases
Anti-FXYD3 antibody [EPR17160] 图像
![Western blot - Anti-FXYD3 antibody [EPR17160] (ab205534)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab205534WBa.jpg)
Western blot - Anti-FXYD3 antibody [EPR17160] (ab205534)
Anti-FXYD3 antibody [EPR17160] (ab205534) at 1/1000 dilution + Human stomach lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 9 kDa
Observed band size : 12 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-FXYD3 antibody [EPR17160] (ab205534)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab205534WBb.jpg)
Western blot - Anti-FXYD3 antibody [EPR17160] (ab205534)
Anti-FXYD3 antibody [EPR17160] (ab205534) at 1/1000 dilution + Human colon cancer lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size : 9 kDa
Observed band size : 12 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-FXYD3 antibody [EPR17160] (ab205534)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab205534WBc.jpg)
Western blot - Anti-FXYD3 antibody [EPR17160] (ab205534)
Anti-FXYD3 antibody [EPR17160] (ab205534) at 1/5000 dilution + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 9 kDa
Observed band size : 12 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXYD3 antibody [EPR17160] (ab205534)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab205534IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXYD3 antibody [EPR17160] (ab205534)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling FXYD3 with ab205534 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane and cytoplasmic staining on human breast carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-FXYD3 antibody [EPR17160] (ab205534)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab205534IFa.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FXYD3 antibody [EPR17160] (ab205534)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling FXYD3 with ab205534 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab205534 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.![Immunocytochemistry/ Immunofluorescence - Anti-FXYD3 antibody [EPR17160] (ab205534)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab205534IFb.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FXYD3 antibody [EPR17160] (ab205534)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells labeling FXYD3 with ab205534 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab205534 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.![Flow Cytometry - Anti-FXYD3 antibody [EPR17160] (ab205534)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab2055344FC.jpg)
Flow Cytometry - Anti-FXYD3 antibody [EPR17160] (ab205534)
Flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling FXYD3 with ab205534 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
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