概述

• 产品名称Anti-Carbonic Anhydrase I抗体[EPR5193]
     

• 描述

  兔单克隆抗体[EPR5193] to Carbonic Anhydrase I

• 经测试应用WB,ICC,IHC-Fr

• 种属反应性

  与反应: Mouse, Rat, Human, Zebrafish    
   

• 免疫原

  A synthetic peptide corresponding to C-terminal residues of Human Carbonic Anhydrase 1

• 阳性对照    

• K562 and TF1 cell lysates

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Alternative versions available:

  Anti-Carbonic Anhydrase I antibody (HRP) [EPR5193] (ab202929)

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR5193

• 同种型IgG

• 研究领域

• Cell Biology

• Other Antibodies

• Other Antibodies

• Cardiovascular

• Blood

• Other

Anti-Carbonic Anhydrase I antibody [EPR5193] 图像

• 

  Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase I [EPR5193] antibody (ab108367)      This image is courtesy of an Abreview submitted by Dr Ryan MacDonald    

     

  ab108367 staining Carbonic Anhydrase I in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab108367 at 1/200 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Carbonic Anhydrase I expression can be observed in horizontal cells and Muller cells(in green).

     

  See Abreview

• 

  Western blot - Carbonic Anhydrase I antibody [EPR5193] (ab108367)          

     All lanes : Anti-Carbonic Anhydrase I antibody [EPR5193] (ab108367) at 1/10000 dilution
  
  Lane 1 : K562 cell lysates
  Lane 2 : TF1 cell lysates
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 29 kDa