详细说明
概述
产品名称Anti-FGD2抗体[EPR15306] - N-terminal
描述
兔单克隆抗体[EPR15306] to FGD2 - N-terminal
经测试应用WB,IHC-P,ICC/IF
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Human FGD2 aa 1-150 (N terminal). The exact sequence is proprietary.
Database link: Q7Z6J4Run BLAST with
Run BLAST with
阳性对照
Human tonsil, Human lymph node and Raji lysates; Human colon tissue; 293T and Raji cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15306
同种型IgG
研究领域
Signal Transduction
Protein Trafficking
Golgi Proteins
Signal Transduction
Protein Trafficking
Organelle Proteins
Signal Transduction
Signaling Pathway
G Protein Signaling
Small G Proteins
Other
Signal Transduction
Cytoskeleton / ECM
Cytoskeleton
Microfilaments
Actin etc
Actin Assembly
Anti-FGD2 antibody [EPR15306] - N-terminal 图像
Western blot - Anti-FGD2 [EPR15306] antibody - N-terminal (ab185968)
All lanes : Anti-FGD2 antibody [EPR15306] - N-terminal (ab185968) at 1/10000 dilution
Lane 1 : Human tonsil lysate
Lane 2 : Human lymph node lysate
Lane 3 : Raji lysate
Lysates/proteins at 20 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 75 kDa
Observed band size : 75 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGD2 [EPR15306] antibody - N-terminal (ab185968)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FGD2 with ab185968 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-FGD2 [EPR15306] antibody - N-terminal (ab185968)
Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling FGD2 with ab185968 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Immunocytochemistry/ Immunofluorescence - Anti-FGD2 [EPR15306] antibody - N-terminal (ab185968)
Immunofluorescent analysis of acetone-fixed Raji cells labeling FGD2 with ab185968 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).