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详细说明
概述
产品名称Anti-Histone H2A (crotonyl K119)抗体[EPR18769]
描述
兔单克隆抗体[EPR18769] to Histone H2A (crotonyl K119)
经测试应用WB,Dot Blot
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H2A aa 100 to the C-terminus (crotonyl K119). The exact sequence is proprietary.
Database link: P04908阳性对照
HeLa whole cell lysate.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR18769
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
Histones
H2A
Crotonylated
Anti-Histone H2A (crotonyl K119) antibody [EPR18769] 图像
![Dot Blot - Anti-Histone H2A (croto<i></i>nyl K119) antibody [EPR18769] (ab195467)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab195467119.jpg)
Dot Blot - Anti-Histone H2A (crotonyl K119) antibody [EPR18769] (ab195467)
Dot blot analysis of H2A K119 crotonyl peptide (Lane 1), H2A K119 ubiquitinate peptide (Lane 2), H2A.X K119 ubiquitinate peptide (Lane 3), H2A K118 formyl peptide (Lane 4), H2A.X unmodified peptide (Lane 5) and H2A unmodified peptide (Lane 6), labeled using ab195467 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H+L (HRP) (ab97051) secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% BSA/TBST.
Exposure time: 3 minutes.
Western blot - Anti-Histone H2A (crotonyl K119) antibody [EPR18769] (ab195467)
Anti-Histone H2A (crotonyl K119) antibody [EPR18769] (ab195467) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 14 kDa
Observed band size : 14 kDa
Exposure time : 30 secondsBlocking/Dilution buffer: 5% BSA/TBST.
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