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详细说明
概述
产品名称Anti-Histone H2B (crotonyl K12)抗体[EPR17593]
描述
兔单克隆抗体[EPR17593] to Histone H2B (crotonyl K12)
经测试应用IHC-P,WB,ICC/IF
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H2B aa 1-100 (crotonyl K12).
Database link: Q16778阳性对照
WB: NIH/3T3 and HeLa whole cell lysates. IHC-P: Human, mouse and rat colon tissues. ICC/IF: HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR17593
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
Histones
H2B
Crotonylated
Anti-Histone H2B (crotonyl K12) antibody [EPR17593] 图像
![Western blot - Anti-Histone H2B (croto<i></i>nyl K12) antibody [EPR17593] (ab177420)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab1774203T3.jpg)
Western blot - Anti-Histone H2B (crotonyl K12) antibody [EPR17593] (ab177420)
All lanes : Anti-Histone H2B (crotonyl K12) antibody [EPR17593] (ab177420) at 1/1000 dilution
Lane 1 : NIH/3T3 whole cell lysates
Lane 2 : HeLa whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 14 kDa
Observed band size : 14 kDa
Blocking/Diluting buffer: 5% NFDM/TBST.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (croto<i></i>nyl K12) antibody [EPR17593] (ab177420)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177420ged.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (crotonyl K12) antibody [EPR17593] (ab177420)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Histone H2B (crotonyl K12) with ab177420 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on glandular epithelium of rat colon tissue is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (crotonyl K12) antibody [EPR17593] (ab177420)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Histone H2B (crotonyl K12) with ab177420 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on glandular epithelium of mouse colon tissue is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (crotonyl K12) antibody [EPR17593] (ab177420)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H2B (crotonyl K12) with ab177420 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on glandular epithelium of Human colon tissue is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (crotonyl K12) antibody [EPR17593] (ab177420)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling Histone H2B (crotonyl K12) with ab177420 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab177420 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.![Peptide Array - Anti-Histone H2B (croto<i></i>nyl K12) antibody [EPR17593] (ab177420)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177420420.jpg)
Peptide Array - Anti-Histone H2B (crotonyl K12) antibody [EPR17593] (ab177420)
All batches of ab177420 are tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
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