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详细说明
概述
产品名称Anti-Histone H2B (crotonyl K5)抗体[EPR17483]
描述
兔单克隆抗体[EPR17483] to Histone H2B (crotonyl K5)
经测试应用IHC-P,WB,ICC/IF
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H2B aa 1-100 (crotonyl K5). The exact sequence is proprietary.
Database link: Q16778
(Peptide available as ab203370)阳性对照
WB: HeLa and NIH3T3 cell lysates;. IHC: Human colon, mouse colon, rat liver tissues. ICC/IF: HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR17483
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
Histones
H2B
Crotonylated
Anti-Histone H2B (crotonyl K5) antibody [EPR17483] 图像
![Western blot - Anti-Histone H2B (croto<i></i>nyl K5) antibody [EPR17483] - N-terminal (ab177396)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177396396.jpg)
Western blot - Anti-Histone H2B (crotonyl K5) antibody [EPR17483] - N-terminal (ab177396)
Anti-Histone H2B (crotonyl K5) antibody [EPR17483] (ab177396) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 14 kDa
Observed band size : 14 kDa
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-Histone H2B (croto<i></i>nyl K5) antibody [EPR17483] - N-terminal (ab177396)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177396962.jpg)
Western blot - Anti-Histone H2B (crotonyl K5) antibody [EPR17483] - N-terminal (ab177396)
Anti-Histone H2B (crotonyl K5) antibody [EPR17483] (ab177396) at 1/500 dilution + NIH/3T3 (Mouse embyro fibroblast cells) cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 14 kDa
Observed band size : 14 kDa
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Peptide Array - Anti-Histone H2B (croto<i></i>nyl K5) antibody [EPR17483] (ab177396)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177396--1.jpg)
Peptide Array - Anti-Histone H2B (crotonyl K5) antibody [EPR17483] (ab177396)
All batches of ab177396 are tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (crotonyl K5) antibody [EPR17483] (ab177396)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H2B (crotonyl K5) using ab177396 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counter stained with Hematoxylin.
Inset image: negative control obtained using PBS instead of ab177396.
Note: Nucleus staining on glandular epithelium of Human colon tissue was observed.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (croto<i></i>nyl K5) antibody [EPR17483] (ab177396)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177396ged.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (crotonyl K5) antibody [EPR17483] (ab177396)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Histone H2B (crotonyl K5) using ab177396 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counter stained with Hematoxylin.
Inset image: negative control obtained using PBS instead of ab177396.
Note: Nucleus staining on glandular epithelium of Mouse colon tissue was observed.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (crotonyl K5) antibody [EPR17483] (ab177396)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Histone H2B (crotonyl K5) using ab177396 at 1/500 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counter stained with Hematoxylin.
Inset image: negative control obtained using PBS instead of ab177396.
Note: Nucleus staining on Rat liver tissue was observed.Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (crotonyl K5) antibody [EPR17483] (ab177396)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H2B (crotonyl K5) with ab177396 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
1. ab177396 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
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