概述

• 产品名称Anti-MPG抗体[EPR10959(B)]
     

• 描述

  兔单克隆抗体[EPR10959(B)] to MPG

• 经测试应用ICC/IF,WB,IHC-P,ICC,IP

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide, corresponding to residues in Human MPG (UniProt: P29372).

• 阳性对照    

• HeLa, 293T and Jurkat cell lysates; Human testis tissue and ovarian carcinoma tissue.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

   

  Alternative versions available:

  Anti-MPG antibody (Alexa Fluor^® 488) [EPR10959(B)] (ab204677)
  Anti-MPG antibody (Alexa Fluor^® 647) [EPR10959(B)] (ab204678)

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR10959(B)

• 同种型IgG

• 研究领域

• Epigenetics and Nuclear Signaling

• DNA / RNA

• DNA Damage & Repair

• Base Excision Repair

Anti-MPG antibody [EPR10959(B)] 图像

• 

  Western blot - Anti-MPG antibody [EPR10959(B)] (ab155092)          

     All lanes : Anti-MPG antibody [EPR10959(B)] (ab155092) at 1/10000 dilution
  
  Lane 1 : HeLa cell lysate
  Lane 2 : 293T cell lysate
  Lane 3 : Jurkat cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat anti-rabbit HRP at 1/2000 dilution
  
  Predicted band size : 33 kDa
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG antibody [EPR10959(B)] (ab155092)          

     Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MPG with ab155092 at 1/50 dilution.  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG antibody [EPR10959(B)] (ab155092)          

     Immunohistochemical analysis of paraffin-embedded Human tesis tissue labeling MPG with ab155092 at 1/50 dilution.  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-MPG antibody [EPR10959(B)](ab155092)          

     Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue using ab155092 showing +ve staining.
  
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-MPG antibody [EPR10959(B)](ab155092)          

     Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155092 showing +ve staining.
  
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-MPG antibody [EPR10959(B)](ab155092)          

     Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155092 showing +ve staining.
  
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-MPG antibody [EPR10959(B)](ab155092)          

     Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155092 showing +ve staining.
  
   

• 

  Immunocytochemistry/ Immunofluorescence - Anti-MPG antibody [EPR10959(B)] (ab155092)      This image is courtesy of an anonymous Abreview    

     

  ab155092 staining MPG in human primary fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

     

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-MPG antibody [EPR10959(B)] (ab155092)      Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada    

     ab155092 (1/500) staining MPG in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5%Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.    

  See Abreview