概述

• 产品名称Anti-Ikaros抗体[EPR13791] - C-terminal
     

• 描述

  兔单克隆抗体[EPR13791] to Ikaros - C-terminal

• 特异性Based on the sequence analysis, ab190691 recognizes seven isoforms with the predicted MWs of 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.

• 经测试应用ICC/IF,WB

• 种属反应性

  与反应: Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Ikaros aa 500 to the C-terminus (C terminal). The exact sequence is proprietary.
  Database link: Q13422

• 阳性对照    

• Jurkat , Raji and Ramos cell line lysate. Jurkat cells.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR13791

• 同种型IgG

• 研究领域

• Cancer

• Cell Death

• Apoptosis

• Other

• Cancer

• Invasion/microenvironment

• Apoptosis

• Other

• Epigenetics and Nuclear Signaling

• Transcription

• Domain Families

• Zinc Finger

Anti-Ikaros antibody [EPR13791] - C-terminal 图像

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  Immunocytochemistry/ Immunofluorescence - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)          

     

  Immunofluorescent analysis of 4% paraformaldehyde fixed Jurkat cells labeling Ikaros with ab190691 at 1/250 followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and conterstained with DAPI.

   

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  Western blot - Anti-Ikaros [EPR13791] antibody - C-terminal (ab190691)          

     All lanes : Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691) at 1/10000 dilution
  
  Lane 1 : Raji cell line lysate
  Lane 2 : Jurkat cell line lysate
  Lane 3 : Ramos cell line lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
  
  Predicted band size : 58 kDa
  Observed band size : 50-70 kDa (why is the actual band size different from the predicted?)