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详细说明
概述
产品名称Anti-NME3抗体[EPR13117]
描述
兔单克隆抗体[EPR13117] to NME3
经测试应用WB,IHC-P,Flow Cyt,IP
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NME3 aa 50-150. The exact sequence is proprietary.
Database link: Q13232阳性对照
MOLT4, Raji and HeLa cell lysates; Human cardiac muscle and kidney tissues; HeLa and MOLT4 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR13117
同种型IgG
研究领域
Metabolism
Pathways and Processes
Metabolic signaling pathways
Energy transfer pathways
Energy Metabolism
Signal Transduction
Metabolism
Energy Metabolism
Signal Transduction
Second Messenger
Nucleotide Messenger
GTP
Anti-NME3 antibody [EPR13117] 图像
![Immunoprecipitation - Anti-NME3 [EPR13117] antibody (ab181257)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18125757e.jpg)
Immunoprecipitation - Anti-NME3 [EPR13117] antibody (ab181257)
Western blot analysis of immunoprecipitation pellet from MOLT4 cell lysate immunoprecipitated using ab181257 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.![Flow Cytometry - Anti-NME3 [EPR13117] antibody (ab181257)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18125757d.jpg)
Flow Cytometry - Anti-NME3 [EPR13117] antibody (ab181257)
Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling NME3 with ab181257 at 1/170 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 [EPR13117] antibody (ab181257)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18125757c.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 [EPR13117] antibody (ab181257)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NME3 with ab181257 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 [EPR13117] antibody (ab181257)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18125757b.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 [EPR13117] antibody (ab181257)
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling NME3 with ab181257 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
![Western blot - Anti-NME3 [EPR13117] antibody (ab181257)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181257257.jpg)
Western blot - Anti-NME3 [EPR13117] antibody (ab181257)
All lanes : Anti-NME3 antibody [EPR13117] (ab181257) at 1/2000 dilution
Lane 1 : MOLT4 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 19 kDa
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