概述

• 产品名称Anti-NAK/TBK1抗体[EP611Y]
     

• 描述

  兔单克隆抗体[EP611Y] to NAK/TBK1

• 经测试应用ICC/IF,WB,IHC-P,ICC

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NAK/TBK1 aa 1-100 (N terminal).

• 阳性对照    

• Hela cells, human breast carcinoma.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

   

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 
  

• 克隆单克隆

• 克隆编号EP611Y

• 同种型IgG

• 研究领域

• Epigenetics and Nuclear Signaling

• Nuclear Signaling Pathways

• NFkB pathway

• Signal Transduction

• Signaling Pathway

• Nuclear Signaling

• NFkB Pathway

• <a javascript:void(0)>Signal Transduction

• Protein Phosphorylation

• Ser / Thr Kinases

• Other Kinases

Anti-NAK/TBK1 antibody [EP611Y] 图像

• 

  Western blot - Anti-NAK antibody [EP611Y] (ab40676)          

     
  
  Predicted band size : 84 kDa
     

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: NAK knockout HAP1 cell lysate (20 µg)
  Lane 3: HeLa cell lysate (20 µg)
  Lane 4: HepG2 cell lysate (20 µg)
  Lanes 1 - 4: Merged signal (red and green). Green - ab40676 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
  ab40676 was shown to specifically react with NAK when NAK knockout samples were used. Wild-type and NAK knockout samples were subjected to SDS-PAGE. ab40676 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000  respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

   

• 

  Anti-NAK antibody [EP611Y] (ab40676)          

     Immunohistochemical analysis of paraffin embedded human breast carcinoma using ab40676 diluted 1:50.    Immunohistochemical analysis of paraffin embedded human breast carcinoma using ab40676 diluted 1:50.  

• 

  Western blot - Anti-NAK antibody [EP611Y] (ab40676)          

     Anti-NAK/TBK1 antibody [EP611Y] (ab40676) at 1/1000 dilution + HeLa cell lysate at 10 µg
  
  Predicted band size : 84 kDa
  Observed band size : 84 kDa
   

• 

  Immunocytochemistry - Anti-NAK antibody [EP611Y] (ab40676)          

     Immunofluorescent staining of HeLa cells using ab40676 diluted 1:500.