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详细说明
概述
产品名称Anti-Histone H4 (crotonyl K8)抗体[EPR17905(R)]
描述
兔单克隆抗体[EPR17905(R)] to Histone H4 (crotonyl K8)
经测试应用IHC-P,WB,ICC/IF
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H4 aa 1-100 (crotonyl K8). The exact sequence is proprietary.
Database link: P62805阳性对照
WB: NIH/3T3, HeLa, C6 and PC-12 whole cell lysates. IHC-P: Mouse colon and rat liver tissues. ICC/IF: HeLa and Neuro-2a cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR17905(R)
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
Histones
H4
Crotonylated
Anti-Histone H4 (crotonyl K8) antibody [EPR17905(R)] 图像
![Western blot - Anti-Histone H4 (croto<i></i>nyl K8) antibody [EPR17905(R)] (ab201075)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201075Rwb.jpg)
Western blot - Anti-Histone H4 (crotonyl K8) antibody [EPR17905(R)] (ab201075)
All lanes : Anti-Histone H4 (crotonyl K8) antibody [EPR17905(R)] (ab201075) at 1/1000 dilution
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
developed using the ECL technique
Predicted band size : 11 kDa
Observed band size : 11 kDa
Exposure time : 30 secondsBlocking/Dilution buffer: 5% BSA/TBST.
![Western blot - Anti-Histone H4 (croto<i></i>nyl K8) antibody [EPR17905(R)] (ab201075)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201075WBb.jpg)
Western blot - Anti-Histone H4 (crotonyl K8) antibody [EPR17905(R)] (ab201075)
All lanes : Anti-Histone H4 (crotonyl K8) antibody [EPR17905(R)] (ab201075) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
developed using the ECL technique
Predicted band size : 11 kDa
Observed band size : 11 kDa
Exposure time : 30 secondsBlocking/Dilution buffer: 5% BSA/TBST.
![Peptide Array - Anti-Histone H4 (croto<i></i>nyl K8) antibody [EPR17905(R)] (ab201075)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab2010755PA.jpg)
Peptide Array - Anti-Histone H4 (crotonyl K8) antibody [EPR17905(R)] (ab201075)
All batches of ab201075 are tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (croto<i></i>nyl K8) antibody [EPR17905(R)] (ab201075)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201075lon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K8) antibody [EPR17905(R)] (ab201075)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Histone H4 (crotonyl K8) with ab201075 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on mouse colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (croto<i></i>nyl K8) antibody [EPR17905(R)] (ab201075)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201075ver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K8) antibody [EPR17905(R)] (ab201075)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Histone H4 (crotonyl K8) with ab201075 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on rat liver is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (croto<i></i>nyl K8) antibody [EPR17905(R)] (ab201075)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201075ged.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (crotonyl K8) antibody [EPR17905(R)] (ab201075)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H4 (crotonyl K8) with ab201075 at 1/1000 dilution, followed by Goat Anti-rRbbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab201075 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (crotonyl K8) antibody [EPR17905(R)] (ab201075)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling Histone H4 (crotonyl K8) with ab201075 at 1/1000 dilution, followed by Goat Anti Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on Neuro-2a cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab201075 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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