概述

• 产品名称Anti-Mutant p53抗体[E47]
     

• 描述

  兔单克隆抗体[E47] to Mutant p53

• 特异性ab32509 recognises human mutant forms of p53 but not human p53 wild type. ab32059 showed a negative signal on wildtype p53 cell lines (HepG2, A549, MCF-7) and a positive signal on mutant p53 cell lines (T47-D, Raji, A431) in WB.

• 经测试应用ICC/IF,WB,ICC,IP,Flow Cyt

• 种属反应性

  与反应: Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Mutant p53 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
  Database link: P04637

• 阳性对照    

• WB: A431 cell lysate.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  A trial size is available to purchase for this antibody.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

  Alternative version available:
  Anti-Mutant p53 antibody (Phycoerythrin) [E47] (ab208992)

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

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• 克隆单克隆

• 克隆编号E47

• 同种型IgG

• 研究领域

• Cancer

• Oncoproteins/suppressors

• Tumor suppressors

• p53 pathway

• Cancer

• Cell cycle

• Cell cycle inhibitors

• p53 pathway

• Epigenetics and Nuclear Signaling

• Chromatin Binding Proteins

• DNA / RNA binding

• Epigenetics and Nuclear Signaling

• Cell cycle

• Cell Cycle Inhibitors

• p53

• Epigenetics and Nuclear Signaling

• Transcription

• Cancer susceptibility

• Tumor Suppressors

• Epigenetics and Nuclear Signaling

• DNA / RNA

• DNA Damage & Repair

• DNA Damage Response

• p53

• Cell Biology

• Apoptosis

• Intracellular

• p53 Pathway

• Cell Biology

• Cell Cycle

• Cell Cycle Inhibitors

• p53

Anti-Mutant p53 antibody [E47] 图像

• 

  Western blot - Anti-Mutant p53 [E47] antibody (ab32509)          

     Anti-Mutant p53 antibody [E47] (ab32509) at 1/1000 dilution + A431 lysate
  
  Predicted band size : 44 kDa
  Observed band size : 53 kDa (why is the actual band size different from the predicted?)