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详细说明
概述
产品名称Anti-hnRNP U抗体[EPR12278(2)(B)]
描述
兔单克隆抗体[EPR12278(2)(B)] to hnRNP U
经测试应用ICC/IF,Flow Cyt,IHC-P,WB
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human hnRNP U aa 50-150. The exact sequence is proprietary.
Database link: Q00839阳性对照
K562, Jurkat and 293 cell lines; Human clear cell carcinoma of kidney tissue.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR12278(2)(B)
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
DNA / RNA
RNA Processing
Other
Anti-hnRNP U antibody [EPR12278(2)(B)] 图像
![Western blot - Anti-hnRNP U [EPR12278(2)(B)] antibody (ab180952)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180952952.jpg)
Western blot - Anti-hnRNP U [EPR12278(2)(B)] antibody (ab180952)
All lanes : Anti-hnRNP U antibody [EPR12278(2)(B)] (ab180952) at 1/20000 dilution
Lane 1 : K562 cell lysate with NFDM/TBST
Lane 2 : Jurkat cell lysate with NFDM/TBST
Lane 3 : 293 cell lysate with NFDM/TBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
Peroxidase conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size : 91 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U [EPR12278(2)(B)] antibody (ab180952)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1809522-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U [EPR12278(2)(B)] antibody (ab180952)
Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney labeling hnRNP U using ab180952 at a 1/100 dilution, with Hematoxylin counter staining. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-hnRNP U [EPR12278(2)(B)] antibody (ab180952)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1809522-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP U [EPR12278(2)(B)] antibody (ab180952)
Immunofluorescence analysis of -20℃ acetone-fixed Jurkat cells labeling hnRNP U using ab180952 at a 1/100 dilution (green) with Dapi counter staining (blue). Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody.
![Flow Cytometry - Anti-hnRNP U [EPR12278(2)(B)] antibody (ab180952)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1809522-4.jpg)
Flow Cytometry - Anti-hnRNP U [EPR12278(2)(B)] antibody (ab180952)
Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling hnRNP U using ab180952 at a 1/100 dilution, or Rabbit monoclonal IgG as an isotype control.
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