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详细说明
概述
产品名称Anti-NDUFB9抗体[EPR15955-78]
描述
兔单克隆抗体[EPR15955-78] to NDUFB9
经测试应用WB,IP,ICC/IF,Flow Cyt,IHC-P
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Human NDUFB9 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q9Y6M9Run BLAST with
Run BLAST with 
阳性对照
WB: Jurkat and HEK293 cell lysates; Human fetal liver lysate; Mouse kidney, mouse spleen, rat heart and rat spleen lysates. IHC-P: Human kidney and mouse cardiac muscle tissue. ICC/IF: Jurkat cells. Flow Cyt: Jurkat cells. IP: Jurkat whole cell lysate.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15955-78
同种型IgG
研究领域
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Oxidative phosphorylation
Complex I
Anti-NDUFB9 antibody [EPR15955-78] 图像
![Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200198WBa.jpg)
Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)
All lanes : Anti-NDUFB9 antibody [EPR15955-78] (ab200198) at 1/5000 dilution
Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate
Lane 2 : HEK293 (Human embryonic kidney) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 22 kDa
Observed band size : 22 kDa
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200198WBb.jpg)
Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)
Anti-NDUFB9 antibody [EPR15955-78] (ab200198) at 1/5000 dilution + Human fetal liver lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 22 kDa
Observed band size : 22 kDa
Exposure time : 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200198WBc.jpg)
Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)
All lanes : Anti-NDUFB9 antibody [EPR15955-78] (ab200198) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat heart lysate
Lane 4 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 22 kDa
Observed band size : 22 kDa
Exposure time : 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200198HCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB9 with ab200198 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Cytoplasm staining on Human kidney tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab200198HCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling NDUFB9 with ab200198 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Cytoplasm staining on mouse cardiac muscle tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab2001988IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NDUFB9 with ab200198 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).
Cytoplasm staining on Jurkat cell line is observed.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab200198 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.![Flow Cytometry - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab2001988FC.jpg)
Flow Cytometry - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NDUFB9 with ab200198 at 1/400 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
![Immunoprecipitation - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab2001988IP.jpg)
Immunoprecipitation - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)
NDUFB9 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200198 at 1/150 dilution.
Western blot was performed from the immunoprecipitate using ab200198 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Jurkat whole cell lysate 10 µg (Input).
Lane 2: ab200198 IP in Jurkat whole cell elysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200198 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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