概述

• 产品名称Anti-LOXL2抗体[EPR12733] - C-terminal
     

• 描述

  兔单克隆抗体[EPR12733] to LOXL2 - C-terminal

• 特异性Our lab has been unable to use this product successfully in IHC-P and so we cannot guarantee that it will work in this application. However, some customers have been successful with this antibody in IHC-P.

• 经测试应用ICC/IF,Flow Cyt,WB

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LOXL2 aa 750 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
  Database link: Q9Y4K0

• 阳性对照    

• Human fetal brain, Human breast cancer, MDA-MB-435, U87-MG and MCF7 lysates; MCF7 and U87-MG cells.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

  If you have any questions regarding this update, please contact our Scientific Support team.

   

  Alternative versions available:

  Anti-LOXL2 antibody (HRP) [EPR12733] - C-terminal (ab202536)

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 49% PBS, 0.05% BSA

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR12733

• 同种型IgG

• 研究领域

• Cancer

• Tumor biomarkers

• Other

• Signal Transduction

• Cytoskeleton / ECM

• Extracellular Matrix

• ECM Enzymes

• Other Enzymes

Anti-LOXL2 antibody [EPR12733] - C-terminal 图像

• 

  Western blot - Anti-LOXL2 [EPR12733] antibody - C-terminal (ab179810)          

     All lanes : Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810) at 1/1000 dilution
  
  Lane 1 : Human fetal brain lysate
  Lane 2 : Human breast cancer lysate
  Lane 3 : MDA-MB-435 lysate
  Lane 4 : U87-MG lysate
  Lane 5 : MCF7 lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 87 kDa
   

• 

  Immunocytochemistry/ Immunofluorescence - Anti-LOXL2 [EPR12733] antibody - C-terminal (ab179810)          

     

  Immunofluorescent analysis of U87-MG cells labeling LOXL2 with ab179810 at 1/100 (red) and DAPI staining (blue).

   

• 

  Flow Cytometry - Anti-LOXL2 [EPR12733] antibody - C-terminal (ab179810)          

     

  Flow Cytometrical analysis of permeabilized U87-MG cells labeling LOXL2 with ab179810 at 1/10 (red) or a rabbit IgG (negative) (green).

   

• 

  Immunocytochemistry/ Immunofluorescence - Anti-LOXL2 [EPR12733] antibody - C-terminal (ab179810)          

     

  Immunofluorescent analysis of MCF7 cells labeling LOXL2 with ab179810 at 1/100 (green) and DAPI staining (blue).