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详细说明
概述
产品名称Anti-Histone H3 (formyl K122)抗体[EPR17896]
描述
兔单克隆抗体[EPR17896] to Histone H3 (formyl K122)
经测试应用WB,IHC-P
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H3 aa 100 to the C-terminus (formyl K122). The exact sequence is proprietary.
Database link: P68431阳性对照
WB: NIH/3T3 and HeLa cell lysates. IHC-P: Human colon, mouse kidney and rat kidney tissues.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR17896
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
Histones
H3
Formylated
Anti-Histone H3 (formyl K122) antibody [EPR17896] 图像
![Western blot - Anti-Histone H3 (formyl K122) antibody [EPR17896] (ab177306)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177306896.jpg)
Western blot - Anti-Histone H3 (formyl K122) antibody [EPR17896] (ab177306)
All lanes : Anti-Histone H3 (formyl K122) antibody [EPR17896] (ab177306) at 1/2000 dilution
Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 15 kDa
Observed band size : 15 kDa
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (formyl K122) antibody [EPR17896] (ab177306)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177306ged.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (formyl K122) antibody [EPR17896] (ab177306)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H3 (formyl K122) with ab177306 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Peptide Array - Anti-Histone H3 (formyl K122) antibody [EPR17896] (ab177306)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab1773066PA.jpg)
Peptide Array - Anti-Histone H3 (formyl K122) antibody [EPR17896] (ab177306)
All batches of ab177306 are tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (formyl K122) antibody [EPR17896] (ab177306)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Histone H3 (formyl K122) with ab177306 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on mouse kidney is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (formyl K122) antibody [EPR17896] (ab177306)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Histone H3 (formyl K122) with ab177306 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on rat kidney is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
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