概述

• 产品名称Anti-Histone H3 (mono methyl K122)抗体[EPR17897]
     

• 描述

  兔单克隆抗体[EPR17897] to Histone H3 (mono methyl K122)

• 经测试应用WB

• 种属反应性

  与反应: Mouse, Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H3 aa 100 to the C-terminus (mono methyl K122) (Cysteine residue). The exact sequence is proprietary.
  Database link: P68431

• 阳性对照    

• HeLa and NIH/3T3 whole cell lysates; HeLa histone extraction lysate; HeLa lysate - G1/S arrested cells (thymidine treatment); HeLa lysate - G2/M arrested cells (sequential thymidine and nocodazole treatments).

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR17897

• 同种型IgG

• 研究领域

• Epigenetics and Nuclear Signaling

• Histones

• H3

• Methylated

Anti-Histone H3 (mono methyl K122) antibody [EPR17897] 图像

• 

  Peptide Array - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300)          

     

  
  All batches of ab177300 are tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
   
  Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
   
  The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

   

• 

  Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300)          

     All lanes : Anti-ASPHD1 antibody - N-terminal (ab177330) at 1/5000 dilution
  
  Lane 1 : HeLa cytosolic fraction
  Lane 2 : HeLa nuclear fraction
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 15 kDa
  Observed band size : 15 kDa
  
  
  Exposure time : 30 seconds    

  Blocking and dilution buffer: 5% BSA/TBST.

   

• 

  Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300)          

     Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300) at 1/5000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) histone extraction lysate at 10 µg
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  developed using the ECL technique
  
  Predicted band size : 15 kDa
  Observed band size : 15 kDa
  
  
  Exposure time : 15 seconds    

  Blocking/Dilution buffer: 5% BSA/TBST.

   

• 

  Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300)          

     Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300) at 1/5000 dilution + NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  developed using the ECL technique
  
  Predicted band size : 15 kDa
  Observed band size : 15 kDa
  
  
  Exposure time : 30 seconds    

  Blocking/Dilution buffer: 5% BSA/TBST.

   

• 

  Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300)          

     All lanes : Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300) at 1/5000 dilution
  
  Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate: untreated, asynchronous cells
  Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate: G1/S arrested cells (thymidine treatment)
  Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate: G2/M arrested cells (sequential thymidine and nocodazole treatments)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  developed using the ECL technique
  
  Predicted band size : 15 kDa
  Observed band size : 15 kDa
  
  
  Exposure time : 30 seconds    

  Blocking/Dilution buffer: 5% BSA/TBST.