概述

• 产品名称Anti-HAUSP / USP7抗体[EPR4253]
     

• 描述

  兔单克隆抗体[EPR4253] to HAUSP / USP7

• 经测试应用WB,IHC-P,ICC/IF

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  A synthetic peptide corresponding to residues in Human HAUSP/ USP7.

• 阳性对照    

• Ramos, HeLa, A431, MCF7, C6 and RAW 264.7 cell lysates; Human colon tissue; HeLa cells.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR4253

• 同种型IgG

• 研究领域

• Epigenetics and Nuclear Signaling

• Ubiquitin & Ubiquitin Like Modifiers

• Deubiquitination

• Cancer

• Cell cycle

• Cell cycle inhibitors

• p53 pathway

• Cell Biology

• Proteolysis / Ubiquitin

• Proteasome / Ubiquitin

• Deubiquitination

• Microbiology

• Interspecies Interaction

• Host Virus Interaction

• <a javascript:void(0)>Cell Biology

• Cell Cycle

• Cell Cycle Inhibitors

• p53

Anti-HAUSP / USP7 antibody [EPR4253] 图像

• 

  Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)          

     
  
  Predicted band size : 128 kDa
     

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: HAUSP/USP7 knockout HAP1 cell lysate (20 µg)
  Lane 3: HeLa cell lysate (20 µg)
  Lane 4: MCF7 cell lysate (20 µg)
  Lanes 1 - 4: Merged signal (red and green). Green - ab108931 observed at 115 kDa. Red - loading control, ab8245, observed at 37 kDa.
  ab108931 was shown to specifically react with HAUSP/USP7 when HAUSP/USP7 knockout samples were used. Wild-type and HAUSP/USP7 knockout samples were subjected to SDS-PAGE. ab108931 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

   

• 

  Western blot - HAUSP / USP7 antibody [EPR4253] (ab108931)          

     All lanes : Anti-HAUSP / USP7 antibody [EPR4253] (ab108931) at 1/1000 dilution
  
  Lane 1 : Ramos cell lysate
  Lane 2 : HeLa cell lysate
  Lane 3 : A431 cell lysate
  Lane 4 : MCF7 cell lysate
  Lane 5 : C6 cell lysate
  Lane 6 : RAW 264.7 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 128 kDa
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HAUSP / USP7 antibody [EPR4253] (ab108931)          

     ab108931, at 1/50, staining HAUSP / USP7 in Human colon tissue by Immunohistochemistry, Paraffin-embedded tissue.  

• 

  Immunocytochemistry/ Immunofluorescence - HAUSP / USP7 antibody [EPR4253] (ab108931)          

     ab108931, at 1/100, staining HAUSP / USP7 in HeLa cells by Immunofluorescence.