概述

• 产品名称Anti-HAUSP / USP7抗体[EPR4254]
     

• 描述

  兔单克隆抗体[EPR4254] to HAUSP / USP7

• 经测试应用WB,IP

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide corresponding to residues in Human USP7 (UniProt Q93009).

• 阳性对照    

• T47-D and Hela cell lysates

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR4254

• 同种型IgG

• 研究领域

• Epigenetics and Nuclear Signaling

• Ubiquitin & Ubiquitin Like Modifiers

• Deubiquitination

• Cancer

• Cell cycle

• Cell cycle inhibitors

• p53 pathway

• Cell Biology

• Proteolysis / Ubiquitin

• Proteasome / Ubiquitin

• Deubiquitination

• Microbiology

• Interspecies Interaction

• Host Virus Interaction

• <a javascript:void(0)>Cell Biology

• Cell Cycle

• Cell Cycle Inhibitors

• p53

Anti-HAUSP / USP7 antibody [EPR4254] 图像

• 

  Western blot - Anti-HAUSP / USP7 antibody [EPR4254] (ab109109)          

     
  
  Predicted band size : 128 kDa
     

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: HAUSP / USP7 knockout HAP1 cell lysate (20 µg)
  Lane 3: HeLa cell lysate (20 µg)
  Lane 4: MCF-7 cell lysate (20 µg) 
  Lanes 1 - 4: Merged signal (red and green). Green - ab109109 observed at 135 kDa. Red - loading control, ab8245, observed at 37 kDa.
  ab109109 was shown to specifically react with HAUSP / USP7 when HAUSP / USP7 knockout samples were used. Wild-type and HAUSP / USP7 knockout samples were subjected to SDS-PAGE. ab109109 and ab8245 (loading control to GADPH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

   

• 

  Western blot - HAUSP / USP7 antibody [EPR4254] (ab109109)          

     All lanes : Anti-HAUSP / USP7 antibody [EPR4254] (ab109109) at 1/1000 dilution
  
  Lane 1 : T47-D cell lysate
  Lane 2 : Hela cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 128 kDa
  Observed band size : 135 kDa (why is the actual band size different from the predicted?)