概述

• 产品名称Anti-Nephrin (phospho Y1176 + Y1193)抗体[EPTPG1]
     

• 描述

  兔单克隆抗体[EPTPG1] to Nephrin (phospho Y1176 + Y1193)

• 特异性This antibody detects Nephrin when either tyrosines 1176 or 1193 are phosphorylated, but does not detect non-phosphorylated Nephrin.

• 经测试应用WB

• 种属反应性

  与反应: Mouse, Human    
   

• 免疫原

  A synthetic peptide corresponding to residues surrounding tyrosine 1176 and 1193 of mouse Nephrin.

• 阳性对照    

• Transfected HEK293T cells

• 常规说明

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液pH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPTPG1

• 同种型IgG

• 研究领域

• <a javascript:void(0)>Signal Transduction

• Cytoskeleton / ECM

• Cell Adhesion

• Cell Adhesion Molecules

• Endothelial

Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] 图像

• 

  Western blot - Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)          

     All lanes : Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299) at 1/50000 dilution
  
  Lane 1 : HEK293T cells transfected with wild type CD 16/7 Nephrin
  Lane 2 : HEK293T cells transfected with active tyrosine kinase (vSrc)
  Lane 3 : HEK293T cells transfected with Nephrin mutated at Tyr1176 and vSrc
  Lane 4 : HEK293T cells transfected with Nephrin mutated at Tyr 1193 and vSrc using Phospho Nephrin (pY1176/1193)
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 138 kDa
  Observed band size : 138,180 kDa (why is the actual band size different from the predicted?)