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详细说明
概述
产品名称Anti-NDUFS3抗体[EPR12781]
描述
兔单克隆抗体[EPR12781] to NDUFS3
经测试应用WB,IHC-P
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NDUFS3 aa 1-100. The exact sequence is proprietary.
Database link: O75489阳性对照
Human fetal heart, Human fetal brain, HepG2, HeLa, C6, Raw264.7, PC-12 and NIH/3T3 cell lysates; Human hepatocellular carcinoma tissue.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR12781
同种型IgG
研究领域
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Oxidative phosphorylation
Complex I
Metabolism
Pathways and Processes
Redox metabolism
Oxidative stress
Metabolism
Pathways and Processes
Metabolic signaling pathways
Energy transfer pathways
Integration of energy
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Mitochondrial markers
Cancer
Cancer Metabolism
Cellular metabolic process
Cancer
Cancer Metabolism
Metabolic signaling pathway
Integration of energy metabolism
Cell Biology
Other Antibodies
Oxidative Stress
Signal Transduction
Metabolism
Mitochondrial
Anti-NDUFS3 antibody [EPR12781] 图像
![Western blot - Anti-NDUFS3 [EPR12781] antibody (ab183733)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1837333WB.jpg)
Western blot - Anti-NDUFS3 [EPR12781] antibody (ab183733)
All lanes : Anti-NDUFS3 antibody [EPR12781] (ab183733) at 1/100000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal brain lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 30 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
![Western blot - Anti-NDUFS3 [EPR12781] antibody (ab183733)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183733WBb.jpg)
Western blot - Anti-NDUFS3 [EPR12781] antibody (ab183733)
All lanes : Anti-NDUFS3 antibody [EPR12781] (ab183733) at 1/20000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 30 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 [EPR12781] antibody (ab183733)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183733IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 [EPR12781] antibody (ab183733)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling NDUFS3 with ab183733 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
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