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详细说明
概述
产品名称Anti-NDUFS3抗体[EPR12782] - C-terminal
描述
兔单克隆抗体[EPR12782] to NDUFS3 - C-terminal
经测试应用WB,IHC-P,ICC/IF,IP
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NDUFS3 aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O75489阳性对照
HepG2, 293T, HL60 and fetal heart lysates. Human heart and kidney tissue. 293 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-NDUFS3 antibody (HRP) - C-terminal [EPR12782] (ab196865)
Anti-NDUFS3 antibody (Alexa Fluor® 488) [EPR12782] - C -terminal (ab197490)
Anti-NDUFS3 antibody (Alexa Fluor® 647) [EPR12782] - C -terminal (ab196864)
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR12782
同种型IgG
研究领域
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Oxidative phosphorylation
Complex I
Metabolism
Pathways and Processes
Redox metabolism
Oxidative stress
Metabolism
Pathways and Processes
Metabolic signaling pathways
Energy transfer pathways
Integration of energy
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Mitochondrial markers
Cancer
Cancer Metabolism
Cellular metabolic process
Cancer
Cancer Metabolism
Metabolic signaling pathway
Integration of energy metabolism
Cell Biology
Other Antibodies
Oxidative Stress
Signal Transduction
Metabolism
Mitochondrial
Anti-NDUFS3 antibody [EPR12782] - C-terminal 图像
![Western blot - Anti-NDUFS3 antibody [EPR12782] - C-terminal (ab177471)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177471458.jpg)
Western blot - Anti-NDUFS3 antibody [EPR12782] - C-terminal (ab177471) Image is courtesy of an anonymous Abreview.
All lanes : Anti-NDUFS3 antibody [EPR12782] - C-terminal (ab177471) at 1/1000 dilution
Lane 1 : Whole cell lysates from NDUFS3 KO cells.
Lane 2 : Whole cell lysates from NDUFS3 KO cells.
Lane 3 : Whole cell lysates from NDUFS3 WT cells.
Lane 4 : Whole cell lysates from NDUFS3 WT cells.
Lane 5 : Isolated mitochondria from NDUFS3 KO Cells.
Lane 6 : Isolated mitochondria from NDUFS3 KO Cells.
Lane 7 : Isolated mitochondria from NDUFS3 WT Cells.
Lane 8 : Isolated mitochondria from NDUFS3 WT Cells.
Lysates/proteins at 50 µg per lane.
Secondary
Goat anti-Rabbit polyclonal HRP conjugate. at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 30 kDa
Exposure time : 1 minuteImage is courtesy of an anonymous Abreview.
See Abreview
![Immunocytochemistry/ Immunofluorescence - Anti-NDUFS3 [EPR12782] antibody (ab177471)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177471icc.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NDUFS3 [EPR12782] antibody (ab177471)
Immunofluorescence analysis of 293 cells labeling NDUFS3 with ab177471 at 1/50 dilution (green). DAPI nuclear staining (blue).
![Immunoprecipitation - Anti-NDUFS3 [EPR12782] antibody (ab177471)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177471-ip.jpg)
Immunoprecipitation - Anti-NDUFS3 [EPR12782] antibody (ab177471)
Western blot analysis on immunoprecipitation pellet from (1) fetal heart lysate or (2) 1XPBS (negative control) using ab177471at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 [EPR12782] antibody (ab177471)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177471hc2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 [EPR12782] antibody (ab177471)
Immunohistochemcal analysis of paraffin-embedded Human heart tissue labeling NDUFS3 with ab177471 at 1/50 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 [EPR12782] antibody - C-terminal (ab177471)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177471711.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 [EPR12782] antibody - C-terminal (ab177471)
Immunohistochemcal analysis of paraffin-embedded Human kidney tissue labeling NDUFS3 with ab177471 at 1/50 dilution.
![Western blot - Anti-NDUFS3 [EPR12782] antibody (ab177471)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177471-wb.jpg)
Western blot - Anti-NDUFS3 [EPR12782] antibody (ab177471)
All lanes : Anti-NDUFS3 antibody [EPR12782] - C-terminal (ab177471) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : 293T lysate
Lane 3 : HL60 lysate
Lane 4 : Fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat-anti-rabbit HRP at 1/2000 dilution
developed using the ECL technique
Predicted band size : 30 kDa
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