概述

• 产品名称Anti-Ndufs4抗体[EP7832]
     

• 描述

  兔单克隆抗体[EP7832] to Ndufs4

• 经测试应用WB,IHC-P,ICC/IF,IP,Flow Cyt

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)

• 阳性对照    

• 293T cell lysates, fetal brain and fetal stomach tissue lysates; Human brain and Human stomach tissues

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20ºC.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EP7832

• 同种型IgG

• 研究领域

• Metabolism

• Pathways and Processes

• Mitochondrial Metabolism

• Oxidative phosphorylation

• Complex I

• Metabolism

• Pathways and Processes

• Metabolic signaling pathways

• Energy transfer pathways

• Integration of energy

• Metabolism

• Pathways and Processes

• Metabolic signaling pathways

• Energy transfer pathways

• Energy Metabolism

• Metabolism

• Pathways and Processes

• Mitochondrial Metabolism

• Mitochondrial markers

• Cancer

• Cancer Metabolism

• Cellular metabolic process

• Cancer

• Cancer Metabolism

• Metabolic signaling pathway

• Integration of energy metabolism

• Neuroscience

• Neurology process

• Neurodegenerative disease

• Other

• Signal Transduction

• Metabolism

• Mitochondrial

• <a javascript:void(0)>Signal Transduction

• Metabolism

• Energy Metabolism

Anti-Ndufs4 antibody [EP7832] 图像

• 

  Western blot - Anti-Ndufs4 antibody [EP7832] (ab137064)          

     
  
  Predicted band size : 20 kDa
     

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: Ndufs4 knockout HAP1 cell lysate (20 µg)
  Lane 3: HEK293 cell lysate (20 µg)
  Lane 4: Human fetal heart tissue lysate (20 µg)
  Lanes 1 - 4: Merged signal (red and green). Green - ab137064 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.
  ab137064 was shown to recognize Ndufs4 when Ndufs4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Ndufs4 knockout samples were subjected to SDS-PAGE. ab137064 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EP7832] (ab137064)          

     Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Ndufs4 with ab137064 at 1/50 dilution.  

• 

  Flow Cytometry - Anti-Ndufs4 antibody [EP7832] (ab137064)          

     Overlay histogram showing HepG2 cells stained with ab137064 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137064, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.  

• 

  Western blot - Anti-Ndufs4 antibody [EP7832] (ab137064)          

     All lanes : Anti-Ndufs4 antibody [EP7832] (ab137064) at 1/1000 dilution
  
  Lane 1 : 293T cell lysate
  Lane 2 : Fetal brain tissue lysate
  Lane 3 : Fetal kidney tissue lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 20 kDa
  Observed band size : 18 kDa (why is the actual band size different from the predicted?)
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EP7832] (ab137064)          

     Immunohistochemical analysis of paraffin-embedded Human stomach tissue labelling Ndufs4 with ab137064 at 1/50 dilution.