Anti-Ndufs4 antibody [EPR7831] 10µl

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Anti-Ndufs4 antibody [EPR7831] 10µl信息二维码

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产品介绍

    基本参数

    详细说明

    概述

    • 产品名称Anti-Ndufs4抗体[EPR7831]
         

    • 描述

      兔单克隆抗体[EPR7831] to Ndufs4

    • 经测试应用WB,IHC-P,IP,Flow Cyt

    • 种属反应性

      与反应: Mouse, Rat, Human    
       

    • 免疫原

      Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

    • 阳性对照    

      • Human fetal heart, Human fetal kidney, Human fetal brain, 293T, Mouse heart, Mouse kidney and Rat kidney lysates; Human kidney and stomach tissues.

    • 常规说明

      This product is a recombinant rabbit monoclonal antibody.

      Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    性能

    • 形式Liquid

    • 存放说明Shipped at 4°C. Store at -20ºC.

    • 存储溶液Preservative: 0.01% Sodium azide
      Constituents: 50% Glycerol, 0.05% BSA

    • 纯度Tissue culture supernatant

    • 克隆单克隆

    • 克隆编号EPR7831

    • 同种型IgG

    • 研究领域

      • Metabolism

      • Pathways and Processes

      • Mitochondrial Metabolism

      • Oxidative phosphorylation

      • Complex I

      • Metabolism

      • Pathways and Processes

      • Metabolic signaling pathways

      • Energy transfer pathways

      • Integration of energy

      • Metabolism

      • Pathways and Processes

      • Metabolic signaling pathways

      • Energy transfer pathways

      • Energy Metabolism

      • Metabolism

      • Pathways and Processes

      • Mitochondrial Metabolism

      • Mitochondrial markers

      • Cancer

      • Cancer Metabolism

      • Cellular metabolic process

      • Cancer

      • Cancer Metabolism

      • Metabolic signaling pathway

      • Integration of energy metabolism

      • Neuroscience

      • Neurology process

      • Neurodegenerative disease

      • Other

      • Signal Transduction

      • Metabolism

      • Mitochondrial

      • <a javascript:void(0)>Signal Transduction

      • Metabolism

      • Energy Metabolism

      Anti-Ndufs4 antibody [EPR7831] 图像

      • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)

        Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)          

           

        Predicted band size : 20 kDa
           

        Lane 1: Wild-type HAP1 cell lysate (20 µg)
        Lane 2: Ndufs4knockout HAP1 cell lysate (20 µg)
        Lane 3: HEK293 cell lysate (20 µg)
        Lane 4: Hu fetal heart tissue lysate (20 µg)
        Lanes 1 - 4: Merged signal (red and green). Green - ab139178 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.
        ab139178 was shown to recognize Ndufs4 when Ndufs4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Ndufs4 knockout samples were subjected to SDS-PAGE. ab139178 and ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

         

      • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)

        Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)          

           All lanes : Anti-Ndufs4 antibody [EPR7831] (ab139178) at 1/1000 dilution

        Lane 1 : Human fetal heart lysate
        Lane 2 : Human fetal kidney lysate
        Lane 3 : Human fetal brain lysate
        Lane 4 : 293T lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        HRP labelled goat anti-rabbit at 2000

        Predicted band size : 20 kDa
         

      • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)

        Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)          

           All lanes : Anti-Ndufs4 antibody [EPR7831] (ab139178) at 1/1000 dilution

        Lane 1 : Mouse heart lysate
        Lane 2 : Mouse kidney lysate
        Lane 3 : Rat kidney lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size : 20 kDa
         

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)          

           Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Ndufs4 with ab139178 at 1/50 dilution.  

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)          

           Immunohistochemical analysis of paraffin embedded Human stomach tissue labelling Ndufs4 with ab139178 at 1/50 dilution.  

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