详细说明
概述
产品名称Anti-MDH1抗体[EPR13597(B)] - C-terminal
描述
兔单克隆抗体[EPR13597(B)] to MDH1 - C-terminal
经测试应用ICC/IF,IP,Flow Cyt,WB
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MDH1 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: P40925阳性对照
Jurlat, HeLa and HepG2 cell lysates; HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR13597(B)
同种型IgG
研究领域
Metabolism
Pathways and Processes
Metabolic signaling pathways
Energy transfer pathways
Energy Metabolism
Metabolism
Pathways and Processes
Metabolic signaling pathways
Carbohydrate metabolism
Cancer
Cancer Metabolism
Metabolic signaling pathway
Metabolism of carbohydrates
Signal Transduction
Metabolism
Energy Metabolism
Anti-MDH1 antibody [EPR13597(B)] - C-terminal 图像
Western blot - Anti-MDH1 [EPR13597(B)] antibody (ab180152)
All lanes : Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152) at 1/50000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 36 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MDH1 [EPR13597(B)] antibody (ab180152)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling MDH1 with ab180152 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution. Counter stained with DAPI.
Immunoprecipitation - Anti-MDH1 [EPR13597(B)] antibody (ab180152)
Western blot analysis of HepG2 cell lysate immunoprecipitated with ab180152 at 1/50 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution
Flow Cytometry - Anti-MDH1 [EPR13597(B)] antibody (ab180152)
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling MDH1 with ab180152 at 1/20 dilution followed by Goat anti rabbit IgG (FITC) at 1/75 dilution (red), compared to a Rabbit IgG control (green).