详细说明
概述
产品名称Anti-GRASP65抗体[EPR12439] - C-terminal
描述
兔单克隆抗体[EPR12439] to GRASP65 - C-terminal
经测试应用ICC/IF,Flow Cyt,IP,IHC-P,WB
种属反应性
与反应: Human
不与反应: Mouse, Rat免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GRASP65 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9BQQ3-1阳性对照
293T, HeLa, HepG2 and MCF7 cell lysates; Human colon and uterus tissues; HepG2 and HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR12439
同种型IgG
研究领域
Cell Biology
Cell Cycle
Cell Division
Other cell division antibodies
Tags & Cell Markers
Subcellular Markers
Organelles
Golgi
Anti-GRASP65 antibody [EPR12439] - C-terminal 图像
Western blot - Anti-GRASP65 [EPR12439] antibody (ab174834)
All lanes : Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
developed using the ECL technique
Predicted band size : 46 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 [EPR12439] antibody (ab174834)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling GRASP65 with ab174834 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 [EPR12439] antibody (ab174834)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue, labeling GRASP65 with ab174834 at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-GRASP65 [EPR12439] antibody (ab174834)
Immunofluorescent analysis of HepG2 cells, labeling GRASP65 with ab174834 at 1/100 dilution (red). DAPI nuclear staining (blue).
Flow Cytometry - Anti-GRASP65 [EPR12439] antibody (ab174834)
Flow cytometry analysis of permeabilized HeLa cells labeling GRASP65 with ab174834 at 1/10 dilution (red), or negative control rabbit IgG (green).
Immunoprecipitation - Anti-GRASP65 [EPR12439] antibody (ab174834)
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling GRASP65, immunoprecipitated using ab174834 at 1/10 dilution.