概述

• 产品名称Anti-Lysyl tRNA synthetase抗体[EPR7921]
     

• 描述

  兔单克隆抗体[EPR7921] to Lysyl tRNA synthetase

• 经测试应用WB,IP,IHC-P,ICC/IF

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide corresponding to residues in Human Lysyl tRNA synthetase (UniProt ID: Q15046).

• 阳性对照    

• Jurkat, HeLa, 293T, HepG2, and Caco 2 cell lysates; Human colon tissue This antibody gave a positive result when used in the following methanol fixed cell lines: CACO-2

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 解离常数（K_D） K_D = 1.44 x 10 ^-11 M Learn more about K_D

• 存储溶液pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.05% BSA

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR7921

• 同种型IgG

• 研究领域

• Epigenetics and Nuclear Signaling

• DNA / RNA

• Translation

• tRNA synthetase

• Epigenetics and Nuclear Signaling

• DNA / RNA

• Translation

• tRNAs

Anti-Lysyl tRNA synthetase antibody [EPR7921] 图像

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Lysyl tRNA synthetase antibody [EPR7921] (ab129080)          

     

  ICC/IF image of ab129080 stained CACO-2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab129080 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

   

• 

  Western blot - Anti-Lysyl tRNA synthetase antibody [EPR7921] (ab129080)          

     All lanes : Anti-Lysyl tRNA synthetase antibody [EPR7921] (ab129080) at 1/1000 dilution
  
  Lane 1 : Jurkat cell lysate
  Lane 2 : HeLa cell lysate
  Lane 3 : 293T cell lysate
  Lane 4 : HepG2 cell lysate
  Lane 5 : Caco 2 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 68 kDa
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysyl tRNA synthetase antibody [EPR7921] (ab129080)          

     ab129080, at a dilution of 1/50, staining Lysyl tRNA synthetase in paraffin embedded Human colon tissue by Immunohistochemistry.  

• 

  Other-Anti-Lysyl tRNA synthetase antibody [EPR7921](ab129080)          

     Equilibrium disassociation constant (K_D)
  Learn more about K_D
  
  Click here to learn more about K_D