• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse JunD in Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human JunD    
  Lys47-Gly156    
  Accession # P17535

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JunD

JunD is a member of the Jun family of nuclear transcription factors. It dimerizes with other Jun or FOS family members to form the AP1 transcription factor. Unlike c-Jun or JunB, JunD antagonizes Ras transformation and accumulates as cells enter quiescence. JunD has two isoforms full length at 347 amino acids (aa), the other 303 aa which initiates translation at aa 44. Over the sequence used as an immunogen, human JunD shares 80% aa identity with mouse and rat JunD. The activation of JunD appears to reduce the proliferation of breast cancer cells.

• Long Name:

  JunD Proto-oncogene

• Entrez Gene IDs:

  3727 (Human); 16478 (Mouse); 24518 (Rat)

• Alternate Names:

  activator protein 1; AP-1; jun D proto-oncogene; JunD; JunD-FL isoform; transcription factor jun-D