• Species Reactivity

  Human

• Specificity

  Detects human Chitobiase/CTBS in direct ELISAs and Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Chitobiase/CTBS    
  Thr39-Arg385    
  Accession # Q01459

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Human Chitobiase/CTBS

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chitobiase/CTBS

CTBS (Di-N-acetylchitobiase; also chitobiase) is a 40 kDa lysosomal glycoprotein that belongs to glycosyl hydrolase family # 18. It is a reducing-end glycoside hydrolase that serves to degrade asparagines-linked carbohydrates. Mature human CTBS is 347 amino acids (aa) in length and contains one catalytic domain (aa 115‑357). There are multiple potential isoforms of CTBS. One shows a three aa deletion of aa 30‑32, accompanied by a two two aa substitution for the C-terminal 279 amino acids. There are two potential alternate start sites, one at Met92 and a second at Met207. Mature human CTBS (aa 39‑385) is 85% aa identical to mouse CTBS.

• Entrez Gene IDs:

  1486 (Human); 74245 (Mouse); 81652 (Rat)

• Alternate Names:

  Chitobiase; chitobiase, di-N-acetyl-; CTBdi-N-acetylchitobiase; CTBS; EC 3.2.1.-