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详细说明
概述
产品名称Anti-Olfactory Marker Protein抗体[EPR19157]
描述
兔单克隆抗体[EPR19157] to Olfactory Marker Protein
经测试应用WB,IHC-P
种属反应性
与反应: Mouse, Rat
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse Olfactory Marker Protein aa 1-100. The exact sequence is proprietary.
Database link: Q64288阳性对照
WB: Mouse OMP full length recombinant protein; Mouse and rat olfactory bulb lysates. IHC-P: Mouse and rat olfactory bulb tissues.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR19157
同种型IgG
研究领域
Neuroscience
Sensory System
Olfactory system
Tags & Cell Markers
Cell Type Markers
Neuroscience Markers
Sensory Neurons
Anti-Olfactory Marker Protein antibody [EPR19157] 图像
![Western blot - Anti-Olfactory Marker Protein antibody [EPR19157] (ab183948)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183948WBa.jpg)
Western blot - Anti-Olfactory Marker Protein antibody [EPR19157] (ab183948)
All lanes : Anti-Olfactory Marker Protein antibody [EPR19157] (ab183948) at 1/1000 dilution
Lane 1 : Rat kidney lysate at 20 µg
Lane 2 : Rat spleen lysate at 20 µg
Lane 3 : Mouse brain lysate at 20 µg
Lane 4 : Mouse heart lysate at 20 µg
Lane 5 : Mouse kidney lysate at 20 µg
Lane 6 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg
Lane 7 : Mouse OMP full length recombinant protein at 0.02 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 19 kDa
Observed band size : 19 kDa
Exposure time : 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.
Uniquely associated with mature olfactory receptor neurons.
Mouse OMP full length recombinant protein contains aa 2-163 with a His-Tag®. The recombinant mouse full length protein was made in-house.![Western blot - Anti-Olfactory Marker Protein antibody [EPR19157] (ab183948)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183948b-2.jpg)
Western blot - Anti-Olfactory Marker Protein antibody [EPR19157] (ab183948)
Lane 1 : Anti-Olfactory Marker Protein antibody [EPR19157] (ab183948) at 1/200 dilution
Lane 2 : Anti-Olfactory Marker Protein antibody [EPR19157] (ab183948) at 1/1000 dilution
Lane 1 : Mouse olfactory bulb lysate
Lane 2 : Rat olfactory bulb lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 19 kDa
Observed band size : 19 kDa
Exposure time : 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.
Uniquely associated with mature olfactory receptor neurons.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein antibody [EPR19157] (ab183948)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183948c-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein antibody [EPR19157] (ab183948)
Immunohistochemical analysis of paraffin-embedded mouse olfactory bulb tissue labeling Olfactory Marker Protein with ab183948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of mouse olfactory bulb is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein antibody [EPR19157] (ab183948)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183948c-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein antibody [EPR19157] (ab183948)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Olfactory Marker Protein with ab183948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on mouse liver. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein antibody [EPR19157] (ab183948)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183948c-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein antibody [EPR19157] (ab183948)
Immunohistochemical analysis of paraffin-embedded rat olfactory bulb tissue labeling Olfactory Marker Protein with ab183948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of rat olfactory bulb is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein antibody [EPR19157] (ab183948)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183948c-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein antibody [EPR19157] (ab183948)
Immunohistochemical analysis of paraffin-embedded rat muscle tissue labeling Olfactory Marker Protein with ab183948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on rat muscle. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
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