概述

• 产品名称Anti-PDGF Receptor alpha (phospho Y720)抗体[EP2478]
     

• 描述

  兔单克隆抗体[EP2478] to PDGF Receptor alpha (phospho Y720)

• 特异性ab134068 only detects PDGF Receptor alpha phosphorylated at Tyrosine 720.

• 经测试应用WB,Flow Cyt

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  A phospho specific peptide corresponding to residues surrounding Tyrosine 720 of Human PDGF Receptor alpha.

• 阳性对照    

• NIH 3T3 cell lysates, treated with Pervanadate.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

   

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液pH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EP2478

• 同种型IgG

• 研究领域

• Cardiovascular

• Atherosclerosis

• Vascular Inflammation

• Inflammatory mediators

• Cancer

• Oncoproteins/suppressors

• Oncoproteins

• Growth factor receptors

• Cancer

• Growth factors

• PDGF

• Signal Transduction

• Growth Factors/Hormones

• PDGF

• Signal Transduction

• Protein Phosphorylation

• Tyrosine Kinases

• Receptor Tyrosine Kinases

• <a javascript:void(0)>Cardiovascular

• Angiogenesis

• Growth Factors

• Other

Anti-PDGF Receptor alpha (phospho Y720) antibody [EP2478] 图像

• 

  Flow Cytometry - Anti-PDGF Receptor alpha (phospho Y720) antibody [EP2478] (ab134068)          

     

  Flow Cytometry analysis of permeabilized NIH/3T3 cells labelling PDGF Receptor alpha (phospho Y720) with ab134068. Green - untreated, Red - pervanadate-treated, Blue - preincubated with phospho-PDGF Receptor alpha (phospho Y720) peptide, Orange - preincubated with non-phospho-PDGR Receptor alpha peptide.

   

• 

  Western blot - Anti-PDGF Receptor alpha (phospho Y720) antibody [EP2478] (ab134068)          

     All lanes : Anti-PDGF Receptor alpha (phospho Y720) antibody [EP2478] (ab134068) at 1/1000 dilution
  
  Lane 1 : NIH 3T3 cell lysate, untreated
  Lane 2 : NIH 3T3 cell lysate, treated with Pervanadate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 122 kDa
  Observed band size : 190 kDa (why is the actual band size different from the predicted?)